【摘要】 目的研究2,3,7,8,-四氯二苯-对-二恶英(2,3,7,8-Tetrachlor odibenzo-p-dioxin,TCDD)对实验性过敏性结膜炎的抑制作用。设计实验研究。研究对象24只雌性BALB/c小鼠随机分成2组,TCDD组和对照组各12只。方法用豚草花粉(Ragweed,RW)对BAL B/c小鼠免疫的前1天,小鼠腹腔内注射1μg TCDD(TCDD组),对照组注射等量的橄榄油,免疫后的第10天用含有RW的溶液点眼攻击,观察眼部临床表现(眼睑充血水肿、流泪),攻击后24小时将小鼠处死,取出眼球及上下眼睑,进行病理分析,计数嗜酸性粒细胞浸润的个数;取出脾脏分离淋巴细胞,分别用特异性抗原Ragweed及Anti-CD3m Ab刺激,通过酶联免疫吸附实验检测细胞因子(IL-4、IL-5、IFN-γ、IL-10)的生成,以3H-Td R掺入法通过液体闪烁计数仪测定每分钟脉冲数值检测细胞增生反应。主要指标结膜炎临床与病理表现(眼睑充血水肿、流泪,嗜酸性粒细胞浸润的个数),IL-4、IL-5、IFN-γ、IL-10细胞因子数,T淋巴细胞增生反应数。结果 TCDD组未发现明显的临床症状,而对照组小鼠出现不同程度流泪、结膜充血等症状。TCDD组脾细胞中未测到IL-4及IL-5生成,对照组IL-4及IL-5分别为(5.34±0.82)pg/ml和(3.56±0.11)pg/ml(t=2.3,P=0.025);但经过Anti-CD3m Ab刺激后TCDD组细胞因子IL-4及IL-5分别为(2.31±0.49)pg/ml和(3.16±0.66)pg/ml,对照组为(2.82±1.12)pg/ml和(4.31±0.89)pg/m l(t=0.15,P=0.078);TCDD组及对照组小鼠脾细胞在经过RW抗原刺激后细胞增生反应分别为(300±13)cpm和(1500±15)cpm(P=0.01),而在抗Anti-CD3m Ab刺激后细胞增生为(15 500±550)cpm和(16 700±670)cpm,差异无统计学意义(P=0.071)。结论 TCDD抑制了小鼠实验性过敏性结膜炎的发展。更多还原

【Abstract】 Objective To investigate the inhibitory effect of TCDD on experimental allergic conjunctivitis(EAC) in mice. Design Experimental study. Participants Twenty-four female BALB/c mice were divided into 2 groups randomly, TCDD group and control group. Methods BALB/c mice were injected 1μg of TCDD 1 day before immunization with ragweed(RW). The control group was injected with equal olive oil. Ten days after immunization, the mice eyes were dropped with RW-containing eye drops. The clinical manifestation of the eyes include eyelid hyperemia and edema, tears were observed. The mice were sacrificed 24 hours latter. The eyes balls and the eyelids were removed for pathological analysis. The number of eosinophil infiltration was counted. The spleens were removed and the lymphocytes were isolated, and stimulated by the specific antigen Ragweed and anti-CD3 m Ab respectively. IL-4, IL-5, IFN-γ, IL-10 were measured by ELISA, and the cell proliferation reaction was detected with 3H-Td R incorporation by liquid scintillation counting pulses per minute value determination apparatus. Main Outcome Measures Clinical manifestation of the eyes include eyelid hyperemia and edema, tears. Expression of cytokines(IL-4, IL-5, IFN-γ, IL-10) and the proliferation response. Results There were no obvious clinical symptoms found in TCDD group. While the control group mice appeared different degree of tears, conjunctival congestion and others. IL-4 and IL-5 didn’t be detected in TCDD group and(5.34±0.82) pg/ml,(3.56 ±0.11)pg/ml in control group respectively(t=2.3, P=0.025). After stimulation with anti-CD3 m Ab, the production of IL-4 and IL-5 were(2.31±0.49) pg/ml,(3.16±0.66) pg/ml in TCDD group and(2.8±1.12) pg/ml,(4.3±0.89) pg/ml in control group(t=0.15, P=0.078). The cell proliferation reaction after RW antigen stimulated were(300±13) cpm and(1500±15) cpm respectively in TCDD group and control group(P=0.01), and(15 500±550) cpm,(16 700±670) cpm after anti-CD3 m Ab stimulation(P=0.071). Conclusions TCDD inhibits the development of experimental allergic conjunctivitis in mice.更多还原