【摘要】 目的 探讨胃癌组织中miR-143、miR-145的表达及其与胃癌临床病理特征的关系。方法 采用Real-time PCR法检测miR-143、miR-145在胃癌组织中的表达，分析miR-143和miR-145的表达与胃癌临床分期、分级的关系。结果 Real-time PCR法检测提示miR-143和miR-145在胃癌组织中较癌旁组织中表达明显降低,差异具有统计学意义(P<0.05)。miR-143和miR-145的低表达与临床分期、有无淋巴结转移及分化程度有关，2种miR的表达与年龄、性别无关。胃癌组织中miR-143、miR-145表达呈正相关(r=0.792,P<0.05)。结论 miR-143和miR-145在胃癌中明显低表达，可能与胃癌的发生密切相关。更多还原

【Abstract】 Objective To investigate the expression of miR-143 and miR-145 in human gastric cancer and its relationship with pathological characteristics. Methods The amounts of miR-143 and miR-145 in gastric cancer were detected by real-time PCR. Meanwhile, their association with clinical stages and grades were analyzed. Results According to real-time PCR, gastric cancer samples presented remarkably reduced levels of miR-143 and miR-145 than normal adjacent tissues. The expression of miR-143 and miR-145 in gastric cancer were correlated with TNM stage, tumor differentiation and lymph node metastasis, rather than to age and gender. The levels of miR-143 and miR-145 was positively correlated in gastric cancer(r=0.792, P<0.05). Conclusion The quantities of miR-143 and miR-145 were significantly decreased in gastric cancer, which may be closely related with the pathogenesis of gastric cancer.更多还原