【摘要】 目的 观察姜黄素预处理对大鼠胃缺血/再灌注(gastric ischemia-reperfusion, GI/R)损伤的保护作用。方法 采用夹闭大鼠腹腔动脉30 min再灌注1 h建立GI/R损伤模型。应用计算机软件分析计算GI/R引起的胃黏膜损伤面积，根据whtie评分法计算胃黏膜损伤指数，用免疫组化法与图像分析技术检测胃黏膜组织B细胞淋巴瘤因子2(Bcl-2)与Bcl-2相关X蛋白(Bax)表达量。结果 与对照组相比，GI/R组胃黏膜损伤面积和损伤指数明显增加(P<0.05,P<0.01);姜黄素预处理能够有效减轻GI/R引起的胃黏膜损伤，随着姜黄素浓度的增加，GI/R引起的胃黏膜损伤面积和损伤指数显著减少，并具有浓度依赖性(P<0.05,P<0.01)。同时，姜黄素预处理能够降低GI/R引起的胃黏膜组织Bax蛋白表达，增加胃黏膜组织Bcl-2蛋白表达，且随着姜黄素浓度的增加，其差异具有浓度依赖性(P<0.05,P<0.01)。结论 姜黄素预处理通过增加抗凋亡蛋白Bcl-2、减少促凋亡蛋白Bax的表达有效抑制GI/R引起的胃黏膜损伤，对胃黏膜起到一定程度的保护作用，对临床缺血/再灌注引起的器官损伤具有潜在的治疗作用。更多还原

【Abstract】 Objective To investigate the protective effects of curcumin pretreatment on the gastric ischemia/reperfusion(GI/R) injury in rats. Methods A rat model of GI/R injury was established through occlusion of the celiac artery in rats for 30 min followed by reperfusion for 60 min. Then the area of damaged gastric mucosa was calculated by software, while the index of gastric mucosal damages was assessed according to the white grading method. The amounts of Bcl-2 and Bax in the tissues of gastric mucosa were detected using immunohistochemical methods and image analysis. Results Compared with the control, rats in the GI/R group presented damages over remarkably larger areas and higher damage indices(P<0.05 or P<0.01). Curcumin pretreatment could relieve the gastric mucosa injury caused by GI/R. The area of gastric mucosal damages and the damage index became significantly lowered with the rising of the concentrations of curcumin(P<0.05 or P<0.01). Meanwhile, curcumin pretreatment could weaken the amounts of Bax but enhance the level of Bcl-2 in the gastric mucosal tissues after GI/R in a dose-dependent manner(P<0.05 or P<0.01). Conclusion Curcumin pretreatment can protect the gastric mucosa and attenuate its damages caused by GI/R through enhancing Bcl-2 but weakening Bax protein expression. This finding indicates the potential role of curcumin as a protective agent against organ injuries caused by ischemia/reperfusion.更多还原