【摘要】 目的 观察小剂量纳洛酮对小鼠皮下一次性注射瑞芬太尼引起痛觉过敏的影响。方法 50只雌性昆明种小鼠随机均分为5组：纳洛酮组（NS组）、瑞芬太尼组（RS组）、瑞芬太尼与各剂量纳洛酮(100 ng·kg^-1、10 ng·kg^-1、1 ng·kg^-1)合用组（RN₁、RN₂、RN₃组）,瑞芬太尼的剂量均为80μg·kg^-1,每组10只。腹腔注射纳洛酮或生理盐水15 min后皮下注射瑞芬太尼，观察比较基础痛阈与给瑞芬太尼后5、20、35、60、90、120、150、180 min对小鼠热板法痛阈（HPPT）的影响。结果 与基础痛阈比较，NS组在5～180 min内HPPT均未见明显改变（P>0.05）;RS组5～35 min内HPPT延长，60 min时HPPT与基础痛阈比较无统计学差异（P>0.05）,而90～120 min内HPPT缩短（P<0.05或P<0.01）;RN₁、RN₂、RN₃组在5～60 min内HPPT延长（P<0.01）;与RS组比较，RN₁、RN₂、RN₃组在5～35 min内HPPT无统计学差异（P>0.05）,但在60～120 min内HPPT均显著增加（P<0.01）。结论 1～100 ng·kg^-1的纳洛酮能缓解甚至避免瑞芬太尼引发的痛觉过敏现象。更多还原

【Abstract】 Objective To observe the effects of low-dose naloxone on hyperalgesia in rats induced by a single subcutaneous injection of remifentail. Methods Fifty female rats were randomly divided into five groups（n=10）, according to their treatment with naloxone（group NS）, remifentail（group RS）, 80 μg/kg remifentail + 100 ng/kg naloxone（group RN1）, 80 μg/kg remifentail + 10 ng/kg naloxone（group RN₂） and 80 μg/kg remifentail + 1 ng/kg naloxone（group RN₃）. Remifentanil was subcutaneously injected 15 min after intraperitoneal administration of naloxone or normal saline. Then, pain threshold in hot-plate test（HPPT） and basal HPPT were compared 5, 20, 35, 60, 90, 120, 150, and 180 min after remifentanil treatment. Results Compared with basal line, no obvious changes were seen as to the HPPT of group NS from 5 to 180 min（P>0.05）. Meanwhile, compared with basal line, group RS produced a higher HPPT from 5 to 35 min, which showed no statistical difference at 60 min（P>0.05）, but then became lower from 90 to 120 min（P<0.05, P<0.01）. The HPPT of groups RN₁, RN₂, and RN₃ was extended from 5 to 60 min（P<0.01） and showed no obvious changes from 90 to 120 min（P>0.05）. When compared with group RS, groups RN₁, RN₂ and RN₃ caused no obvious differences in HPPT from 5 to 35 min（P>0.05）, which however was significantly longer from 90 to 120min（P<0.01）. Conclusion Naloxone can alleviate or even prevent remifentanil-induced hyperalgesia at doses of 1 to 100 ng/kg.更多还原