【摘要】 目的:研究HS6101对小鼠造血细胞的动员作用。方法:对正常ICR小鼠1或2次皮下注射HS6101 9μg/鼠、单剂量1次皮下注射HS6101 3、9、27和81μg/鼠后分别观察HS6101不同给药次数和不同给药剂量的动员效果;并比较单剂量HS6101加rhG-CSF联合给药(rhG-CSF给药剂量为2μg/d/鼠,连续5 d皮下注射)的协同作用。观察各组动物给药后不同时间外周血象及药后5、10 d骨髓和外周血中造血干细胞数。结果:HS6101 9μg/鼠1次和2次皮下注射给药对外周血细胞计数的影响无显著差别。HS6101 3、9、27和81μg/鼠使ICR小鼠外周血小板数呈剂量依赖性升高,药后5 d检测值分别为PBS对照组的121.1%、138.7%、146.4%和156.2%(r=0.777),7 d检测值分别为118.0%、123.1%、139.2%和168.7%(r=0.954)。HS6101 27μg/鼠药后5 d可明显增加骨髓和外周血中造血干细胞数,升高外周血白细胞数。结论:HS6101对正常ICR小鼠造血干细胞、血小板和白细胞有动员作用。更多还原

【Abstract】 Objective: This study was aimed to investigate the mobilization effect of HS6101 on hematopoietic cells of mice. Methods: The normal ICR mice were injected subcutaneously once or twice with HS6101 at 9 μg /d /mouse,or a single dose of HS6101 3,9,27 and 81 μg /mouse was administrated,and the mobilization effect of HS6101 in different administration times and different dosage was observed, and compared with the synergistic effects of administration of single dose of HS6101 combined with rhG-CSF( 2 μg /d /mouse was injected subcutaneously for 5consecutive days). The peripheral blood cell counts of mice were detected at different time after administration. The hematopoietic stem /progenitor cells of bone marrow and peripheral blood were detected at day 5 and 10 after administration.Results: There was no significant difference in peripheral blood cell counts after once or twice injections of HS6101 9 μg /mouse. The peripheral platelet counts dose-dependently increased in ICR mice,which accounted for121. 1% to 118. 0%,138. 7% to 123. 1%,146. 4% to 139. 2%,and 156. 2% to 168. 7%( P < 0. 001) after HS6101( 3,9,27 and 81 μg /mouse) treatments at 5 and 7 d,respectively. HS6101( 3,9,27 and 81 μg /mouse) showed dose-response relationship to platelets,with R value of 0. 777 and 0. 954 at day 5 and 7 after administration,respectively.HS6101 significantly increased numbers of hematopoietic stem /progenitor cells in both bone marrow and peripheral blood,and elevated peripheral blood leukocytes at 27 μg /mouse dose at day 5 after administration. Conclusion: HS6101 has significant mobilization effect on hematopoietic stem /progenitor cells,platelets and leukocytes in mouse.更多还原