【摘要】 目的:本研究旨在探讨多发性骨髓瘤细胞形态及细胞免疫表型特点。方法:采用流式细胞仪多参数直接免疫荧光技术、CD45/SSC配合CD38++/CD138+设门,对47例多发性骨髓瘤细胞标记CD138、CD38、CD56、CD117、CD3、CD13、CD33、CD19、CD7、CD20、CD22、CD34、CD28进行检测,并结合骨髓细胞形态检查进行分析。结果:流式细胞分析应用CD45/SSC配合CD38+/CD138+设门证实多发性骨髓瘤患者可疑瘤细胞比例为9.42%-74.25%,形态学检查骨髓瘤细胞比例为11.0%-80.6%,两种方法之间有良好的正相关性(r2=0.54,P<0.001);抗原阳性表达比例分别为:CD138为74.46%,CD38为100%,CD56为57.44%,CD117为40.42%,CD13为6.38%,CD33为19.15%,CD20为8.51%,CD28为27.66%,CD22为2.12%,CD34为4.25%,CD3为0.00%,CD19为0.00%,CD7为0.00%。结论:CD45/SSC配合CD38+/CD138+设门技术可以比较准确地设定需要分析的多发性骨髓瘤细胞群,瘤细胞大多数表达CD138、CD38、CD56抗原;免疫表型分析结合细胞形态学检查,有助于多发性骨髓瘤的诊断和鉴别诊断。更多还原

【Abstract】 Objective:This study was to investigate the cell morphology and cell immune phenotypic characteristics in patients with multiple myeloma（MM）.Methods:The flow cytometry with multiparametric direct immunofluorescence technique,and CD45/SSC and CD38^{+ +} /CD138⁺ gating were used to measure cell markers CD138,CD38,CD56,CD117,CD3,CD13,CD33,CD19,CD7,CD20,CD22,CD34,CD28 in 47 MM patients.At the same time the morphology examination of bone marrow cells was performed.Results:The suspicious myeloma cell ratio in MM patients was 9.42%-74.25%detected by flow cytometry,moreover,the myeloma cell ratio detected by morphology examination was 11.0%-80.6%,there was a good correlation between the two detection methods（r = 0.54,P <0.001）.The ratio of antigen positive expression was as follows:74.46%for CD138,100%for CD38,57.44%for CD56,40.42%for CD117,6.38%for CD13,19.15%for CD33,8.51%for CD20,27.66%for CD28,2.12%for CD22,4.25%for CD34,0%for CD3,0%for CD 19,0%for CD7.Conclusions:CD45/SSC and CD38⁺ /CD138⁺gating technique can accurately gate multiple myeloma cell sets which need analysis,the majority of myeloma cells expreses CD138,CD38,CD56 antigens.The immunophenotypic analysis combined with the cell morphology examination more contribute to the diagnosis and differential diagnosis of multiple myeloma.更多还原