The present study aimed to investigate the targeting effect of H7K(R2)2-modified pH -sensitive liposomes on U87-MG cells. Using coumarin-6 as a fluorescence probe, we prepared H7K(R2)2-modified p H-sensitive liposomes(designated as coumarin-6-PSL-H7K(R2)2). The flow cytometry assay was used to evaluate the effect of H7K(R2)2 proportions on the cellular uptake and endocytosis pathways of coumarin--6--PSL--H7K(R2)2 on U87-MG cells. The circular dichroism(CD) spectroscopy assay was used to investigate the seco...
【基金】
Specialized Research Fund for the Doctoral Program of Higher Education(Grant No.20120001110012);
the National Basic Research Program of China(973 Program,Grant No.2013CB932501);
National Natural Science Foundation of China(Grant No.81172992)
【更新日期】
2015-10-29
【分类号】
R96
【正文快照】
1.IntroductionThe growth of solid tumors is characterized by theuncontrolled proliferation of cancer cells and by thechanges in the tumor environment such as acidic p H[1].The typical tumor extracelllar p H(p He)in most solidtumors is reported at the rang