【摘要】 目的建立同时测定紫朱软膏中紫草的左旋紫草素和β,β’-二甲基丙烯酰阿卡宁含量的高效液相色谱方法。方法采用Welch Materials XB-C18色谱柱(4.6 mm×250 mm,18.5μm),流动相乙腈-水-甲酸(75∶25∶0.05),检测波长516 nm,柱温30℃,流速1.0 mL/min,进样量20μL,对紫朱软膏中紫草的有效成分左旋紫草素和β,β’-二甲基丙烯酰阿卡宁进行定量测定。结果左旋紫草素在0.025 6~0.256μg范围内线性关系良好(r2=0.999 5),平均回收率为98.12%(RSD=1.92%);β,β’-二甲基丙烯酰阿卡宁在0.174 4~2.616μg范围内线性关系良好(r2=0.999 2),平均回收率为100.46%(RSD=1.17%)。结论本试验建立的方法简便,测定结果准确,可用于紫朱软膏的质量控制和评价。更多还原

【Abstract】 Objective To establish an HPLC method to simultaneously determine the contents of shikonin and β,β’-dimethylacrylshikonin in Zizhu Ointment. Methods The contents of shikonin and β,β’-dimethylacrylshikonin in Zizhu Ointment were determined by HPLC. The chromatographic conditions were Welch Materials XB-C18 Column(4.6 mm × 250 mm, 18.5 μm), with mobile phase of acetonitrile-water-formic acid(75∶25∶0.05), detection wavelength of 516 nm, temperature of 30 ℃, flow rate of 1.0 mL /min and the load of 20 μL. Results Shikonin showed a good linearity in the range of 0.025 6-0.256 μg(r 2=0.999 5), and the average recovery rate was 98.12%(RSD=1.92%). β,β’-dimethylacrylshikonin showed a good linearity in the range of 0.174 4-2.616 μg(r 2=0.999 2), and the average recovery rate was 100.46%(RSD=1.17%). Conclusion This method is simple and accurate, which can be used for the quality control and evaluation of Zizhu Ointment.更多还原