【摘要】 为了从分子水平揭示野生鼠类血原体的种类特征和系统发生关系,无菌采集32份野生白腹巨鼠血样,抽提全血基因组DNA,采用真细菌的通用引物进行16SrRNA基因的扩增,对扩增产物进行克隆和测序。结果从其中21只血样中成功地扩增出目的片段大小的核苷酸序列。对阳性产物进行克隆、测序,获得了两条代表性序列(GenBank登录号HQ183731和HQ183732)。序列分析显示,获得的两条序列与GenBank中收录的鼠Mycoplasma haemomuris 16SrRNA基因(AB758435)相似性最高,分别为95%和97%。两个样本间16SrRNA基因序列相似性达98.3%。种系发育分析表明,获得白腹巨鼠血原体的两条序列形成了独立的进化分支,并与来自野鼠的Haemobartonella muris(HMU82963)和来自家鼠M.haemomuris(AB758435)所形成的进化分枝为姊妹枝。上述研究证明,白腹巨鼠具有较普遍的M.haemomuris感染,并与已报道的啮齿动物M.haemomuris有一定的遗传差异,是一种新基因型的血原体。对进一步研究人和动物的亲血性支原体的流行病学、种群生物学等研究具有一定价值。更多还原

【Abstract】 This experiment was conducted to study the species characteristic and phylogenetic relationship of hemoplasmas from the wild rats on molecular level.Thirty-two blood samples collected from wild Rattus edwardsi in Hunan and Guangxi province of China were investigated by PCR aplication based on the 16 SrRNA gene sequence.The total infection rate of hemoplasmas was 65.63%(21/32).Genetic variations showed that the inter-specific differences between M.haemomuris isolates and other Mycoplasmaspecies(3.0%-4.5%)were bigger than that(0.8%)between two synonymic species(Haemobartonella muris and M.haemomuris).Phylogenetic analyses indicated that M.haemomuris derived from wild Rattus edwardsi grouped into a solitary clade closely related to H.muris and M.haemomuris.The results suggested that M.haemomurisisolated in the present study should represent a new genotype,and temporarily named M.haemomuris Rattus edwardsi strain.These data may have important implications for researching epidemiology and population biology as well as for studying the taxonomy and status of the genus Mycoplasma.更多还原