【摘要】 旨在研究褪黑素对妊娠牦牛子宫的调控作用,采用real-time PCR法和免疫组织化学S-P法,对未妊娠和妊娠不同时期牦牛子宫中褪黑素受体(MT1)mRNA和褪黑素受体(MT1)蛋白的表达进行研究。结果表明:牦牛未妊娠和妊娠的不同时期,牦牛子宫基质细胞(SC)和肌层平滑肌细胞(MM)中均有MT1蛋白表达(P>0.05);血管内皮细胞(EBV)和血管平滑肌细胞(VSM)中的MT1蛋白表达,在妊娠28~35d差异不显著,随妊娠时间的增加,MT1蛋白表达增加(P<0.05);腺上皮细胞(GE)中的MT1蛋白,在妊娠初期(妊娠28~35d)差异不显著,妊娠50~60d时,差异显著(P<0.05),妊娠90~100d时,差异极显著(P<0.01)。而在腔上皮细胞(SE)中,MT1蛋白表达量在妊娠28~35d比未妊娠时低(P<0.05),而这种趋势随妊娠时间的增加继续降低(P<0.01)。未妊娠牦牛子宫中MT1mRNA表达量显著高于妊娠牦牛(P<0.01)。综上表明,在妊娠期,牦牛子宫对褪黑素的需求量降低,褪黑素主要作用于牦牛GE,调节子宫腺功能,使牦牛子宫产生适应妊娠的生理反应。更多还原

【Abstract】 To better understand the role of melatonin receptor(MT1)during pregnancy of yak,the expression level of MT1 protein and MT1 mRNA in uterus of yak(Bos grunniens)during pregnant and nonpregnant stage was determined using Streptavidin-Peroxidase(S-P)immunohistochemical method and real-time PCR,respectively.The results showed that MT1 protein expressed in stromal cells and myometrial smooth muscle cells during pregnancy and nonpregnant stage(P>0.05).The expression level of MT1 protein in endometrial blood vessel and vascular smooth muscle had no significant difference on 28-35 day of pregnancy compared with that of nonpregnant yak,however,it was increasing with the stage of pregnancy(P<0.05).Meanwhile,MT1 protein expression was not significant different on 28-35 day of pregnancy in gland epithelium,but had dramatic increases on 50-60 day of pregnancy(P<0.05)and 90-100 day of pregnancy(P<0.01).In surface epithelium cells,the expression of MT1 protein was lower on 28-35 day of pregnancy than that of nonpregnant stage(P<0.05)and had significant decreases on 50-60 day of pregnancy(P<0.01)and 90-100 day of pregnancy(P<0.01).In addition,MT1 mRNA expression was lower in pregnant uterus than that of nonpregnant stage(P<0.01).The results in-dicated that the decreased MT1 mRNA expression in uterus during pregnancy might be associated with gland epithelium cell function for maintenance of pregnancy.更多还原