【摘要】 目的比较第2代杂交捕获技术(HC-2)和低温PCR检测法对宫颈病变的诊断应用价值。方法收集2013年4月-2014年4月来新疆医科大学附属肿瘤医院就诊的232例维吾尔族宫颈癌、宫颈上皮内瘤样病变(CIN)及慢性宫颈炎患者宫颈分泌物标本,分别进行HC-2和低温PCR检测,低温PCR技术检测不同宫颈病变组织中HPV亚型,HC-2法测定病变组织中HPV DNA含量。分析2种方法检测结果的一致性。结果在232例标本中,低温PCR检测HPV阳性76例,阳性率为32.76%;HC-2检测HPV阳性70例,阳性率为30.17%。2种方法检测结果一致者210例,一致率为90.51%,Kappa值为0.780;慢性宫颈炎患者HPV亚型以HPV16、HPV56为主;CINⅠ患者HPV亚型以HPV16、HPV18、HPV59为主;CINⅡ患者HPV亚型以HPV16、HPV59为主;CINⅢ患者HPV亚型以HPV16、HPV31为主;宫颈癌患者HPV亚型以HPV16、HPV18、HPV58为主;CINⅠ~Ⅲ以及宫颈癌患者的HPV DNA含量均高于慢性宫颈炎患者(P<0.01),宫颈癌患者的HPV DNA含量高于CINⅠ~Ⅲ,差异均有统计学意义(P<0.01)。结论 HC-2和低温PCR检测在宫颈严重病变中有较强的一致性,2种检测方法对宫颈病变的筛查均有较好的应用价值。更多还原

【Abstract】 Objective To compare the diagnostic and applied value of hybrid capture 2 technology( HC-2) and low temperature PCR assay in cervical lesion. Methods Specimens of cervical secretions from 232 cases of cervical cancer,cervical intraepithelial neoplasia lesions( CIN) and chronic cervicitis were collected in our hospital. The specimens were detected by HC-2 and low temperature PCR assay and the consistency of the results of two methods was analyzed. Results The HPV positive rate of low temperature PCR detection was 32. 76%( 76 /232) and HPV positive rate of HC-2 was 30. 17%( 70 /232) in specimens of 232 cases. The consistent rate of two methods was90. 51%( 210 /232) and the Kappa value was 0. 780; The main HPV subtypes of chronic cervicitis were HPV16,HPV56,and the main HPV subtypes of CINⅠ were HPV16,HPV18,HPV59,and the main HPV subtypes of CINⅡ were HPV16,HPV59,and the main HPV subtypes of CINⅢ were HPV16,HPV31,while the main HPV subtypes of cervical cancer were HPV16,HPV18,HPV58; The HPV DNA levels of CIN Ⅰ-Ⅲ and cervical cancerwere higher than chronic cervicitis( P < 0. 01),in addition,The HPV DNA levels of cervical cancer were higher than CINⅠ-Ⅲ( P < 0. 01). Conclusion The consistency of HC-2 and low temperature PCR detection in cervical severe lesions was strong and the two kinds of detection methods both had strong application value for the screening of cervical lesions.更多还原