【Objective】The study aims to establish several RNA extraction methods which might be of high quality and suitability for Southern Xinjiang five featured fruit trees in the hope of paving the way for subsequent research.【Method】The kit method,traditional CTAB,modified borax-CTAB and improved borax-CTAB-isopropanol RNA isolation methods were used to isolate total RNA from walnut buds,pear buds,apple buds,apricot leaves and almond leaves,and the corresponding results were compared in this study.【Result】Fertili...