【摘要】 目的分析HIV抗体确证实验结果符合率,为提高实验室HIV抗体检测技术提供依据。方法按照《全国艾滋病检测技术规范》(2009版),对大连市2012—2013年初筛送检的1 119份阳性样品采用免疫印迹法(WB)进行确认。结果 (1)经确证实验,阳性率75.43%,不确定率15.46%,阴性率9.20%。(2)确证阳性的,男性占94.66%,女性占5.34%;未婚、已婚、离异分别占55.04%、24.08%、20.88%;29岁及以下占43.65%,30~39岁占24.08%,40~49岁占18.51%,50~59岁占9.37%,60岁及以上占4.39%;小学及以下、初中、高中、大专及大专以上分别占7.24%、24.44%、28.71%、39.62%。(3)WB带型种类分布,出现9条带的占40.69%,8条带占27.28%,7条占22.42%,6条占5.22%,5条占4.39%。(4)确证阳性的,env蛋白、pol蛋白、gag蛋白的平均阳性率分别为98.62%、94.74%、72.04%;不确定的,分别为46.32%、2.46%、36.14%。(5)WB带型年龄分布,3种env蛋白,5个年龄组差异均无统计学意义;3种gag蛋白差异均有统计学意义(P<0.05);p55,60岁及以上组明显低;p17,50~59岁组较高;3种pol蛋白,差异均有统计学意义(P<0.01),3种抗体的阳性率,50~59岁组均为100%,60岁及以上组均明显低。(6)不确定的样本完成追踪检测的96人,其中,转为阳性49人,占51.04%;转为阴性的23人,占23.96%。结论 HIV感染者男性多、未婚多、高学历多、低龄多,在大学要加强同性恋教育。不确定者的带型绝大多数不含抗pol基因编码的抗体。env蛋白与年龄关联小,gag蛋白与年龄有关联,pol蛋白与年龄关联明显。更多还原

【Abstract】 Objective To understand the coincidence rate of HIV antibody in the screening and confirmed tests,so as to provide a basis for improving the HIV antibody examination technology. Methods According to the National HIV/AIDS Test Technical Criterion( 2009 version),1 119 HIV antibody positive serum samples of screening tests were reconfirmed by using Western blot( WB). Results Of the 1 119 samples of screening tests,75. 43% were confirmed to be HIV antibody positive by WB,15. 46% were negative,and 9. 20% were uncertain. Of the WB confirmed positive samples,94. 66% were males and 5. 34% were females; 55. 04% were the unmarried,24. 08% were the married,and 20. 88% were the divorce. The constituent ratios were 43. 65%,24. 08%,18. 51%,9. 37% and 4. 39% in the age groups of less than 29,30- 39,40- 49,50-59,and above 60 years,respectively; The constituent ratios were 7. 24%,24. 44%,28. 71% and 39.62% in the education level groups of primary school or bellow,junior middle school,senior middle school,and college and above,respectively. In the distribution of WB banding patterns,there were 40. 69% with 9 strips,27. 28% with 8 strips,22. 42% with 7 strips,5. 22% with 6 strips,and 4. 39% with 5 strips. Among all the PCS,the average occurrence rates of env,pol and gag were 98. 62%,94. 74%,and 72. 04% respectively,while among the unidentified samples( US),the rates of env,pol and gag were 46. 32%,2. 46%,and 36. 14% respectively. Among the 5 groups divided by age,there were no significant differences for env( gp160,gp120,gp41); but there were significant differences for gag( p55,p24,p17)( all P < 0. 05); and there were significant differences for pol( p66,p51,p31)( all P < 0. 001). Of the 173 US,only 96 were followed up,49 were confirmed to be positive,23 were negative and 24 were still uncertain. Conclusions Among HIV infections,the males,unmarried,highly educated,and younger age were in the majority. We should pay more attention to male college students of homosexuality. Among most of the US,their strips do not contain the pol. The env has no relevance with the age,but the pol and gag have relevance with the age.更多还原