【摘要】 背景:随着基因工程以及肿瘤生物分子学等新兴学科的发展壮大,基因治疗肿瘤成为一种新的治疗模式。目的:探讨ABCE1基因沉默对人宫颈癌XB1702细胞的生长、增殖及迁移等方面的影响。方法:设计及合成ABCE1的siR NA序列,LipofectamineTM 2000转染XB1702细胞。以转染NC siR NA载体细胞为对照组,未转染的宫颈癌XB1702细胞为空白组。通过RT-PCR、Western blot检测RNA干扰后ABCE1 mR NA和蛋白的表达,流式细胞仪检测细胞周期,并通过CCK-8增殖实验、划痕愈合实验、细胞侵袭实验评价沉默ABCE1基因对人宫颈癌XB1702细胞增殖、迁移以及侵袭能力的影响。结果与结论:实验组ABCE1 mR NA和蛋白表达明显低于对照组和空白组(P<0.05)。实验组细胞的生长速度明显减慢,细胞被阻滞在G0/G1期,S期细胞数减少。与对照组和空白组相比,实验组XB1702细胞的增殖受到明显抑制、迁移能力和侵袭能力显著下降(P<0.05)。结果表明特异性干扰ABCE1基因表达可抑制人宫颈癌XB1702细胞的迁移能力,并抑制肿瘤细胞增殖,因此,ABCE1的siR NA序列可能成为治疗宫颈癌的有效靶点。更多还原

【Abstract】 BACKGROUND;With the development of genetic engineering and tumor molecular biology,gene therapy has acted as a new treatment mode for tumors.OBJECTIVE;To investigate the impact of gene silencing ABCE1 on growth,proliferation and migration of human cervical cancer cells XB1702.METHODS;si RNA sequences of ABCE1 were designed and synthesized to transfect XB1702 cells using LipofectamineTM 2000.Cells transfected with NC si RNA carrier served as controls,and untransfected cells acted as blank group.After RNA interference,the expression of ABCE1 m RNA and protein was detected by western blod assay; cell cycle was detected using flow cytometry; cell counting kit-8 test,scratch healing assay and cell invasion assay were used to evaluate the effects of ABCE1 silence gene on XB1702 cell proliferation,migration and invasion ability.RESULTS AND CONCLUSION;ABCE1 m RNA and protein expression in the experimental group was significantly lower than that in the control group and blank group(P < 0.05).Growth rate in the experimental group was significantly slowed,cells were arrested in G0/G1 phase,and the number of S-phase cells was reduced.Compared with the control group and blank group,XB1702 cell proliferation was inhibited significantly in the experimental group,and cell migration and invasion was significantly decreased(P < 0.05).These results showed that specific interference with gene expression of ABCE1 can inhibit the migration of human cervical cancer cells XB1702,and restrain tumor cell proliferation; therefore,the si RNA sequence of ABCE1 may be an effective target for treatment of cervical cancer.更多还原