【摘要】 背景:尽管应用一种干细胞进行组织修复已取有重大进展,但联合两种或多种干细胞构建组织工程骨的研究尚不多见。目的:观察脂肪干细胞联合骨髓间充质干细胞/异体骨植入兔腰椎后路横突间植骨融合模型的骨修复效果。方法:新西兰大白兔75只,随机分为5组,分别在各组兔L5、L6腰椎横突融合模型中做以下处理:1植入骨髓间充质干细胞/异体骨复合骨条,周围注入脂肪干细胞悬液。2植入骨髓间充质干细胞/异体骨复合骨条,周围注入生理盐水。3单纯植入异体骨条,周围注入脂肪干细胞悬液。4单纯植入异体骨条,周围注入生理盐水。5植入自体骨条。术后1,3,5周用PET/CT对各组动物行全身显像,比较各组植骨区SUV值。结果与结论:各组植骨区PET/CT图像均显示了不同程度的骨融合和骨代谢增强。各组植骨区SUV值随时间增加而增高,但单纯异体骨组3个时间点的SUV值差异无显著性意义(P>0.05),其他各组第3周和第5周时SUV值均高于第1周(P<0.05),且第3周与第5周差异无显著性意义(P>0.05)。术后第3周,脂肪干细胞联合骨髓间充质干细胞/异体骨组的SUV值优于骨髓间充质干细胞/异体骨组和自体骨组,差异有显著性意义(P<0.05),说明联合脂肪干细胞和骨髓间充质干细胞快速构建组织工程骨具有良好的成骨和血管化作用。更多还原

【Abstract】 BACKGROUND: Despite tissue repair with a kind of stem cells has achieved great outcomes, but the combination of two or more kinds of stem cells to construct tissue-engineered bone is still rare. OBJECTIVE: To evaluate the efficacy of tissue-engineered bone constructed based on combining adipose-derived stem cells with bone marrow mesenchymal stem cells and allogenic bone in rabbit posterior intertransverse lumbar interbody fusion models.METHODS: Seventy-five New Zealand rabbits were randomly divided into five groups. L5,6 interbody fusion models were made in each group. Then, these five groups were respectively treated as follows(1) group A: bone marrow mesenchymal stem cells/allograft iliac bone with adipose-derived stem cells;(2) group B: bone marrow mesenchymal stem cells/allograft iliac bone with normal saline;(3) group C: allograft iliac bone with adipose-derived stem cells;(4) group D: allograft iliac bone with normal saline; group E: autologous iliac bone alone. Each group was detected by PET/CT to contrast the standard uptake value at 1, 3, 5 weeks postoperatively.RESULTS AND CONCLUSION: PET/CT images showed varying degrees of bone fusion and bone metabolism enhancement in the five groups. With time, the standard uptake value was increased in each group, and except the group D, the standard uptake value was higher in all the groups at 3, 5 weeks than at 1 week(P < 0.05). There was no difference in the standard uptake value of all the five groups at weeks 3 and 5 after operation(P > 0.05). The standard uptake value of the group A was better than that in the group B and group E(P < 0.05). Taken together, the combination of adipose-derived stem cells and bone marrow mesenchymal stem cells for rapid production of tissue-engineered bone has good osteogenesis and vascularization.更多还原