【摘要】 背景:干细胞移植存在多种途径,目前还无法确定哪一种是最佳途径。而对于不同的脑损伤个体,所移植的细胞种类、移植途径和移植时间都将会影响到治疗效果。目的:探讨不同途径移植骨髓单个核细胞对脑损伤大鼠神经功能的影响。方法:Ficoll淋巴细胞分离液梯度离心分离大鼠骨髓单个核细胞,CFDA-SE体外标记后备用;自由落体法制备大鼠创伤性脑损伤模型,模型制作成功后,立即通过损伤区、侧脑室和颈内动脉移植CFDA-SE标记的骨髓单个核细胞,每一种移植途径都设对照组(以等体积的DMEM代替骨髓单个核细胞)。治疗后不同时间点进行m NSS评分,最后一次行为学评分完毕取脑组织,荧光倒置显微镜下观察骨髓单个核细胞在损伤区域的存活和迁移情况。结果与结论:治疗后7,10,14 d,对照组和移植组mN SS评分均较1 d和3 d时降低(P<0.05);治疗后7 d和10 d,颈内动脉移植组和对照组相比mN SS评分降低(P<0.05);治疗后14 d,颈内动脉移植组荧光细胞数量较其他组多(P<0.05),且分布广。结果表明经颈内动脉移植骨髓单个核细胞能明显改善大鼠神经功能,且移植细胞能够在损伤区大量存活和迁移。更多还原

【Abstract】 BACKGROUND: There are several routes for stem cell transplantation; however, it is still unable to determine which one is the best. As for the different individuals with brain injury, the type of transplanted cells, transplantation route and time will affect the therapeutic effects.OBJECTIVE: To investigate the effect of bone marrow mononuclear cells transplanted via different approaches on neurological function of rats with traumatic brain injury. METHODS: Bone marrow mononuclear cells of rats were administered gradient centrifugation with Ficoll lymphocyte separation medium, and were labeled with CFDA-SE in vitro as standby. Rat models of traumaticbrain injury were established by the method of freefall. After successful establishment of rat models, bone marrow mononuclear cells labeled with CFDA-SE were immediately transplanted into rats via injured area, lateral ventricle and internal carotid artery. One control group was designated for each transplantation route(bone marrow mononuclear cells were replaced with the same volume of DMEM). The degree of neurological deficits was evaluated using m NSS scores at different time points after treatment. The brain tissue was harvested after the last neurobehavioral evaluation. The survival and migration of bone marrow mononuclear cells in the injured area were observed under an inverted fluorescent microscope. RESULTS AND CONCLUSION: At 7, 10, and 14 days after treatment, the m NSS scores of rats in all groups were all lower than those at 1 and 3 days(P < 0.05). At 7 and 10 days, the m NSS scores of rats in the internal carotid artery transplantation group were significantly lower than those in the control group(P < 0.05). At 14 days after treatment, the number of fluorescence-labeled cells of rats in the internal carotid artery transplantation group was greater than that in the other groups(P < 0.05) and these labeled cells were widely distributed. The results demonstrate that the neurological function of rats can be improved by transplanting bone marrow mononuclear cells via the internal carotid artery, and a large number of transplanted cells can survive and migrate in the injured area.更多还原