【摘要】 背景:骨量不足限制了口腔种植修复的广泛应用,如何促进干细胞的迁移、黏附和增殖进而促进内源骨再生成为研究的关键点。目的:观察不同生长因子对体外培养兔脂肪干细胞迁移、黏附和增殖的影响,筛选出最佳的组合因子。方法:无菌切除兔腹股沟处的白色脂肪组织,采用酶消化法培养脂肪干细胞,取第3代细胞分为5组进行干预,分别为转化生长因子β1+血小板源性生长因子AB组(组1),血小板源性生长因子AB+血管内皮生长因子组(组2),转化生长因子β1+血管内皮生长因子组(组3),转化生长因子β1+血小板源性生长因子AB+血管内皮生长因子组(组4)和空白对照组。采用Transwell小室法检测细胞的迁移能力,黏附实验检测细胞的黏附能力,CCK-8法检测细胞的增殖能力。结果与结论:Transwell实验显示,4组间迁移细胞数差异有显著性意义(P<0.05),其中,转化生长因子β1(2μg/L)+血小板源性生长因子AB(10μg/L)+血管内皮生长因子(10μg/L)组(组4)迁移细胞数最多,显著促进了脂肪干细胞的迁移。黏附实验结果显示,4组间黏附细胞数差异有显著性意义(P<0.05),其中转化生长因子β1+血管内皮生长因子组(组3)黏附细胞数最多,显著促进了脂肪干细胞的黏附。CCK-8结果显示,在不同的时间点(1,3,5,7 d),各因子组合组吸光度值较对照组均显著增高(P<0.05),其中血小板源性生长因子AB+血管内皮生长因子组(组2)递增的吸光度值最大,显著促进了兔脂肪干细胞的增殖。更多还原

【Abstract】 BACKGROUND:Bone deficiency restricts the extensive use of oral implant restoration.How to promote stem cell migration,adhesion and proliferation so as to promote endogenous bone regeneration becomes the key of research.OBJECTIVE:To observe the effects of different growth factors on the migration,adhesion and proliferation of rabbit adipose tissue-derived stem cells cultured in vitro and to screen the optimal combined factors.METHODS:The inguinal adipose tissues of rabbits were cut under aseptic conditions and primary adipose tissue-derived stem cells were cultured by enzyme digestion method.Passage 3 cells were randomly divided intofive groups:transforming growth factor β1+platelet-derived growth factor AB group(group 1),platelet-derived growth factor AB+vascular endothelial growth factor group(group 2),transforming growth factor β1+vascular endothelial growth factor group(group 3),transforming growth factor β1+platelet-derived growth factor AB+vascular endothelial growth factor group(group 4),and blank control group.In vitro cell migration ability was examined by Transwell chamber.Cell proliferation and adhesion abilities were examined by cell counting kit-8 and cell adhesion assay,respectively.RESULTS AND CONCLUSION:Results from the Transwell test showed that the migration number of rabbit adipose tissue-derived stem cells showed significant differences among the former four groups(P < 0.05),which was highest in the group 4 that significantly promoted the migration of adipose tissue-derived stem cells.In the cell adhesion assay,there were significant differences in the number of adherent cells among the former four groups(P < 0.05),and the group3 showed the most adherent cells that dramatically improved the adhesion of adipose tissue-derived stem cells.Compared with the blank control group,the absorbance values in the groups 1-4 were significantly higher at days 1,3,5,7(P < 0.05),and the group 2 showed the highest absorbance value,indicating the combination of platelet-derived growth factor AB and vascular endothelial growth factor significantly promoted the proliferation of rabbit adipose tissue-derived stem cells.更多还原