【摘要】 目的建立激素非依赖性前列腺癌裸鼠模型,评价京尼平的抗肿瘤作用,并探讨其可能的治疗机制。方法采用颈背部皮下种植肿瘤细胞的方法建立激素非依赖性前列腺癌裸鼠模型,随机分为京尼平低、中、高剂量组及对照组;分别给予20 mg/kg、40 mg/kg、80mg/kg京尼平灌胃及生理盐水灌胃;测量裸鼠体质量、肿瘤体积并计算抑瘤率,流式细胞术检测细胞的增殖和凋亡情况。聚合酶链反应检测解偶联蛋白2(UCP2)的基因表达情况。结果各组裸鼠肿瘤体积在干预前比较差异均无统计学意义(P均>0.05);京尼平能显著抑制肿瘤生长,并随着给药浓度的增高,肿瘤生长抑制率、增殖抑制作用及凋亡率逐渐增高;京尼平能抑制肿瘤细胞中UCP2的表达,中、高剂量组UCP2的基因及蛋白的表达显著低于低剂量组及对照组(P均<0.05)。结论京尼平对激素非依赖性前列腺癌裸鼠模型有明显的抑瘤作用,其作用机制可能与抑制UCP2的表达有关。更多还原

【Abstract】 Objective After successfully constructed nude mice model of androgen independent prostate cancer( AIPC),it is to assess the tumor killing effect of genipin and the possible mechanism. Methods Nude mice model of androgen independent prostate cancer was established by planting tumor cells under the skin of neck and back,then the models were randomly divided into low,medium and high dose of genipin groups and control group,which were given 20,40 and 80 mg / kg genipin solution and normal saline respectively by gavage once a day. Weights of nude mice,tumor volumes were measured to calculate the inhibition rate of AIPC. Proliferation and apoptosis of AIPC models were observed by flow cytometry. Expression of UCP2 was tested by PCR. Results There was no significant difference in tumor volumes among every group before intervention( P > 0. 05). Genipin could significantly inhibit the tumor growth,and the rate of tumor growth inhibition,inhibition in proliferation,apoptosis rate were gradually increased with the increasing of genipin dosage( P < 0. 05). Genipin could inhibit the expression of UCP2 in the models,and the expression in the middle and high dose groups were significantly lower than that in low dose group and control group( P < 0. 05). Conclusion Genipin has significant anti-tumor effect on the AIPC models,and the mechanism may be related to the inhibition of expression of UCP2.更多还原