【摘要】 建立了高效液相色谱—蒸发光检测器(HPLC—ELSD)法检测甲基葡萄糖苷含量的方法。最佳色谱分析条件为:Alltech Apollo C18色谱柱(4.6 mm×250 mm,5μm),流动相V(水)∶V(甲醇)=5∶95,流速为0.7 m L/min,柱温为30℃。ELSD检测器漂移管温度为104℃,载气(空气)流速为2.8 L/min。甲基葡萄糖苷在2.5~12.5μg/μL之间具有良好的线性关系,r=0.999 1,回收率为100.0%~106.3%。该方法简便易行,分析速度快,具有良好的重复性和精密度,适合用于甲基葡萄糖苷的检测。更多还原

【Abstract】 HPLC equipped with ELSD detector is used to determine the content of methyl glucoside. The optimal detection conditions are determined as follows: HPLC instrument fixed with Alltech Apollo C18 column( 4. 6 × 250 mm,5 μm),the mobile phase water-methanol( 5∶ 95,v / v) pumped at a flow rate of 0. 7 m L / min and 30℃ of the operating temperature of column. Drift tube temperature is set at 104℃ and the gas( air) flow rate is 2. 8 L / min. The calibration curve is linear( r = 0. 999 1) within the range of 2. 5- 12. 5 μg / μL for methyl glucoside. The recoveries are in the range from 100. 0% to 106. 3%. This method is simple,accurate,highly sensitive and reproducible,which is suitable for the determination of methyl glucoside.更多还原