【Objective】This study estimated the optimal conditions for total flavonoids extraction from Fertility-promoting intrauterine infusion liquid based on response surface methodology to provide theoretical foundation for its further processing and utilization.【Method】The Fertility-promoting intrauterine infusion liquid was prepared by decoction and alcohol precipitation.On the basis of single factor test,response surface methodology was applied to optimize the extraction conditions including water(mL)-feed(g)ra...