【摘要】 目的观察支气管哮喘小鼠肺组织Th9细胞数量及其相关细胞因子水平,了解它们与支气管哮喘发病的关系。方法16只SPF级雌性BALB/c小鼠按随机数字表法分为正常对照组和哮喘组,每组8只。建立哮喘模型后,2组小鼠分别用小动物无创肺功能仪行气道反应性检测;ELISA检测支气管肺泡灌洗液(BALF)中白细胞介素9(IL-9)、IL-10、IL-13水平;HE染色及PAS染色观察肺组织病变情况;流式细胞术检测肺脏IL-9+、IL-10+、IL-13+淋巴细胞占CD3+淋巴细胞百分数。结果哮喘组气道反应性明显高于正常组,气道炎症明显;BALF中IL-9、IL-13水平较正常组明显升高,IL-10明显降低;肺脏IL-9+、IL-13+淋巴细胞占CD3+淋巴细胞百分数明显高于正常组,IL-10+淋巴细胞占CD3+淋巴细胞百分数明显低于正常组。结论支气管哮喘小鼠肺内IL-9+细胞数量增加、IL-9水平升高,IL-10表达减少,Th9细胞可能在哮喘发病中发挥一定作用。更多还原

【Abstract】 Objective To investigate the number of T helper 9(Th9) cells and the level of Th9 cell related cytokines in the lung tissues of mice with bronchial asthma and understand their relationship to the pathogenesis of bronchial asthma.Methods Sixteen SPF BALB / c mice were randomized into normal group and asthma group(8 mice in each group). After asthmatic models were established,the airway responsiveness was detected with non-invasive lung function machine,ELISA was used for determining the concentrations of cytokine interleukin-9( IL-9),IL-10 and IL-13 in bronchoalveolar lavage fluid(BALF),HE staining and PAS staining was employed for observing the lung pathological changes,flow cytometry was used for testing the percentages of lung IL-9+lymphocytes,IL-10+lymphocytes and IL-13+lymphocytes in lung CD3+lymphocytes. Results Compared with normal group,asthma group showed higher airway responsiveness,more severe airway inflammation and increased concentrations of cytokine IL-9 and IL-13,decreased concentration of cytokine IL-10 in BALF,higher percentages of lung IL-9+lymphocytes and IL-13+lymphocytes in CD3+lymphocytes,and lower percentage of IL-10+lymphocytes. Conclusion The expression of IL-9 was higher,but IL-10 was lower in asthma group. Th9 cel s might play an important role in the pathogenesis of asthma.更多还原