【摘要】 目的研究抗肿瘤药物阿糖胞苷(Ara-C)与顺铂(DDP)联用,或单独使用抗肿瘤药物,作用于具有顺铂耐药性的TW03/DDP鼻咽癌细胞,探讨细胞生长抑制和凋亡的差异及可能机制。方法体外培养TW03/DDP细胞,运用实时定量PCR和Western blot法检测细胞中肺耐药相关蛋白(LRP)的表达;Ara-C与DDP联用或单独使用处理TW03/DDP细胞和不具耐药性的TW03鼻咽癌细胞后,运用CCK-8法检测其生长抑制率,流式细胞术检测其凋亡率。结果相对于TW03细胞,TW03/DDP细胞中LRP蛋白表达升高;Ara-C与DDP单独使用均能抑制TW03/DDP细胞和TW03细胞的生长并诱导凋亡,但DDP对TW03/DDP细胞作用弱;Ara-C与DDP联用可抑制以上两种细胞的生长,抑制率和凋亡率均大于二者单独应用的效果。结论Ara-C与DDP联用可以增强对耐药性鼻咽癌细胞的生长抑制作用和凋亡诱导作用。更多还原

【Abstract】 Objective To investigate the synergistic cytotoxicity of cisplatin and cytosine arabinoside( Ara-C) on nasopharyngeal carcinoma( NPC) cell lines TW03 and cisplatin-resistant TW03/ DDP which is cisplatin-induced drug-resistant cell line. Methods The TW03/ DDP cells was established by gradually increasing the dose of cisplatin,and then examined by real-time quantitative PCR( qRT-PCR) and semi-quantitative Western blotting for the expression level of lung resistancerelated protein( LRP). Effects of DDP and Ara-C combined treatment or alone on proliferation and apoptosis of TW03/ DDP cells and TW03 cells were detected by CCK-8 assay and flow cytometry,respectively. Results Compared with TW03 cells,TW03/ DDP cells expressedhigher level of LRP. After treatments for 24hours,both cisplatin and Ara-C inhibited proliferation and caused apoptosis of TW03/ DDP and TW03 cells,and DDP showedminor effect on the proliferation of TW03/ DDP cells.Compared with Ara-C or DDP treatment alone,combination of Ara-C and DDP showed a more remarkable inhibition of proliferation and promotion of apoptosis in both TW03 and TW03/ DDP cells. Conclusion Ara-C and DDPhave a synergistic killing effect on NPC cell lines with and without DDP-caused drug resistance,and show better effects on proliferation inhibition and apoptosis promotion than Ara-C or DDP treatment alone,which provides a novel and prospective strategy for NPC chemotherapy.更多还原