【摘要】 目的研究Wnt5a/JNK信号通路相关分子在哮喘大鼠肺组织中的表达。方法将实验大鼠随机分为正常组和哮喘组,成功复制哮喘模型后,通过HE染色观察气道病理改变,实时荧光定量PCR检测肺组织和血液中Wnt5a mRNA的表达,Western blot法检测肺组织中磷酸化的c-Jun(p-c-Jun),磷酸化的c-Jun N端激酶(JNK)蛋白的表达。结果与正常组相比,哮喘组支气管黏膜增厚,褶皱增多,管腔狭窄;气管和血管周围有炎细胞的浸润。气道壁与平滑肌层显著变厚。哮喘组大鼠肺组织和血液中Wnt5a mRNA与对照组比较表达上调,p-c-Jun,p-JNK蛋白表达增加。结论哮喘大鼠的Wnt5a、p-JNK、p-c-Jun表达增强。更多还原

【Abstract】 Objective To observe the expression of Wnt5a/c-Jun N-terminal kinase( JNK) signaling pathway in the lung of asthmatic rats. Methods The experimental rats were randomly divided into two groups,the model group and the control group. After the rat models of asthma were established,airway pathological changes were observed by HE staining; the expression of Wnt5a mRNA in the lung tissues and peripheral blood cells was examined by real-time quantitative PCR; and the levels of phospho-c-Jun( p-c-Jun) and phospho-c-Jun N-terminal kinase( p-JNK) proteins in the lung tissues were measured by Western blotting. Results Compared with the control group,asthmatic rats presented with thickened bronchial mucosa with more obvious chrysanthemum fold,narrower airway,and more inflammatory cells infiltrated around trachea and pulmonary vessels. The inner airway wall and smooth muscle layer were much thicker in rats of asthma group than those of control group. The levels of Wnt5 a mRNA,p-c-Jun and p-JNK proteins in the lung and blood of asthmatic rats increased as compared with the control group. Conclusion The expressions of Wnt5 a mRNA,p-JNK and p-c-Jun proteins were elevated in asthmatic rats.更多还原