【摘要】 目的探讨胡桃醌对宫颈鳞癌Si Ha细胞增殖及凋亡的影响。方法选取对数生长期的Si Ha细胞将其分为空白对照组和(10、20、50、80、100)μmol/L胡桃醌组。采用MTT法观察胡桃醌对Si Ha细胞增殖的抑制作用,并计算出半数抑制浓度(IC50),依据IC50确定胡桃醌的有效浓度;annexin V-FITC/PI双染色结合流式细胞术测定20μmol/L胡桃醌对Si Ha细胞凋亡的影响,Western blot法检测凋亡相关蛋白Bcl-2及Bax的表达。结果 MTT试验显示,与对照组比较,各胡桃醌浓度对细胞增殖均有明显抑制作用,IC50为20.4μmol/L;流式细胞术检测表明,正常生长的Si Ha细胞早期凋亡率为(2.46±0.37)%,经20μmol/L的胡桃醌处理细胞12 h,早期凋亡率增加至(18.47±2.26)%;与正常对照组相比,Western blot法检测显示20μmol/L胡桃醌使细胞中Bcl-2表达明显降低而Bax表达明显增加。结论胡桃醌可以抑制Si Ha细胞的增殖,并诱导细胞凋亡。更多还原

【Abstract】 Objective To explore the effect of juglone on proliferation and apoptosis of human cervical squamous cancer Si Ha cells. Methods Cultured Si Ha cells in the exponential growth phase were grouped into blank control group and 10,20,50,80 and 100 μmol / L juglone treatment groups. Methyl thiazolyl tetrazolium( MTT) assay was adopted to observe the inhibitory effect of juglone on the proliferation of Si Ha cells,and then 50% inhibitory concentration( IC50) was calculated through formula. Annexin V-FITC / PI double staining and flow cytometry were used to detect the effect of 20 μmol / L juglone on Si Ha cell apoptosis. Western blot was applied to determine the expressions of Bcl-2 and Bax. Results MTT assay showed that,compared with the control group,treatment groups all showed significant inhibitory effects on Si Ha cell growth,and IC50 was 20. 4 μmol / L. Flow cytometry demonstrated that early apoptosis rate of Si Ha cells in the control group was( 2. 46 ± 0. 37) %,and after treatment with 20 μmol/L Juglone for 12 hours,the apoptosis rate was raised to( 18. 47 ±2. 26) %; Western blot analysis showed that the expression of Bcl-2 decreased while the expression of Bax increased significantly in Si Ha cells treated with 20 μmol / L juglone. Conclusion Juglone could significantly inhibit the proliferation and induce the apoptosis of Si Ha cells.更多还原