【摘要】 目的:研究人血小板裂解液(HPL)对羊膜来源间充质干细胞(AMMSCs)成骨分化的作用。方法:分别以含7%HPL(HPL组)和10%胎牛血清(FBS,FBS组)的LG-DMEM培养介质扩增AMMSCs,比较两组扩增AMMSCs效率及其免疫表型SSEA-3和SSEA-4的表达差异。使用MSCs成骨分化培养液诱导AMMSCs成骨,对比观察两组钙盐沉积量、钙化结节、碱性磷酸酶(ALP)活性;提取两组成骨诱导后AMMSCs总RNA,采用RTPCR检测成骨分化调节因子RUNX-2和ALP mRNA相对表达量。结果:HPL组扩增速度快于FBS组;AMMSCs的SSEA-3和SSEA-4表达较FBS组明显下调(P<0.05)。HPL组钙盐沉积量、钙化结节数量、ALP活性以及RUNX-2和ALP mRNA表达量均明显高于FBS组(P均<0.05)。结论:含HPL培养介质可促进AMMSCs成骨分化。更多还原

【Abstract】 Objective:To investigate the effect of human platelet lysate(HPL)on amnion-derived mesenchymal stem cells(AMMSCs)osteogenic differentiation.Method:AMMSCs were amplified by LG-DMEM medium supplemented with 7% HPL(HPL group)or 10% fetal bovine serum(FBS,FBS group).The surface molecular markers potential SSEA-3 and SSEA-4 were compared.When AMMSCs were used for osteogenic differentiation,ALP activity and calcium deposition were comparatively observed.Total RNA was isolated and the gene expression of RUNX-2 and ALP were investigated by RT-PCR.Results:HPL-containing medium significantly accelerated MSCs proliferation as compared with FBS containing medium.Expressions of embryonic stem cell markers SSEA-3 and SSEA-4 on AMMSCs were promoted down-regulation by HPL-containing medium than that of FBS-containing medium.HPL-containing medium significantly improved ALP activity and calcium deposition,which also enhanced the mRNA level of RUNX-2 and ALP(P<0.05).Conclusion:HPL-containing medium promotes osteogenic differentiation of AMMSCs.更多还原