【摘要】 检测并分析杰出有氧耐力表型与运动应激适应的表观遗传学基因调控模式特征的关联。方法:递增负荷法检测33名现役国家级耐力项目运动员和58名体育教育专业大学生(二级运动员)的最大摄氧量、个体乳酸阈,同步采用microarray及qRT-PCR法检测训练诱导的c-miRNA表达谱,分析杰出耐力表型与训练诱导的miRNA及去乙酰化酶(SIRT1)的基因表达调控特征的关联。结果:杰出耐力表型差异表达27条c-miRNA,18条上调,9条下调,其靶基因富集于AMPK、PPAR、MAPK及SIRT1信号通路,miRNA的调控作用使AMPK通路及下游通路活性上调,SIRT1的表达和活性水平显著上调并增强AMPK通路活性。结论:运动应激诱导的miRNA-SIRT1的调控特征与杰出有氧耐力表型存在关联,对杰出耐力表型的形成具有促进作用,可作为预测有氧耐力发展潜力的表观遗传学分子标记应用于运动选材。更多还原

【Abstract】 The objective of the study was to explore the relationship between the elite endurance phenotype and epigenetics gene regulating effect of miRNA-SIRT1 in exercise stress adaption.33 active elite athletes(national grade)and 58 student athletes(grade 2)were engaged in this test.VO2 max and ILT were measured.Differential expression profiles of c-miRNAs induced by exercise were detected.Targetscan,miRanda,and miRBase were used to predict target genes of those miRNA,and pathway analysis was performed for target gene by KEGG and IPA.Result:There were 27c-miRNAs which regulated the pathway of AMPK,PPAR,MAPK,SIRT1,and their regulating characters were validated to promote the formation of the elite endurance.It could be concluded that there was relationship between miRNA-SIRT1 and exercise stress adaption,the profile of c-miRNAs induced by exercise could be used as an epigenetic biomarker on forecasting and valuing the potential of aerobic endurance.更多还原