【摘要】 目的探讨不同水平下的酪蛋白和小麦面筋蛋白对血浆同型半胱氨酸(Hcy)水平的影响。方法健康Wistar大鼠48只随机分为6组,分别为12.5%、25%、50%酪蛋白组和14.5%、29%、58%小麦面筋蛋白组。大鼠自由进食、饮水,每天称重并记录大鼠进食量。喂养实验饲料14天后处死,采集血液,取肝脏组织,用于测定血浆和肝脏中Hcy、半胱氨酸(Cys)等氨基酸指标以及同型半胱氨酸甜菜碱甲基转移酶(BHMT)和胱硫醚β-合成酶(CBS)等酶学指标。结果小麦面筋蛋白组的大鼠体重增长显著低于酪蛋白组,而在小麦面筋蛋白组随着蛋白含量的提高,大鼠的饲料摄入量明显逐渐减少。小麦面筋蛋白的摄入使血浆Hcy浓度显著低于酪蛋白组,相反血浆Cys的浓度,小麦蛋白组明显高于酪蛋白组。结论小麦面筋蛋白对血浆Hcy水平的影响主要取决于高Cys与低蛋氨酸含量,但是其赖氨酸和苏氨酸含量较低也是不能忽视的影响因素之一。其主要的作用机制是Cys的升高促进了Hcy代谢的酶活性,增加了Hcy的代谢消耗。更多还原

【Abstract】 Objective To investigate the effects of different level of casein and wheat gluten on decreasing plasma homocysteine concentration in rats. Methods 48 rats of the Wistar were fed with different level of casein( 12. 5%,25% and 50%) and wheat gluten( 14. 5%,29% and 58%) diets for 14 days,and they were killed by decapitation to obtainblood and livers was subject to analysis the concentration of homocysteine,cysteine and other amino acids,as well as BHMT and CBS activities. Results Body weight gain in rats fed wheat gluten dietary was significantly less than casein dietary,but food intake was significantly decreased in wheat gluten group with increasing of the protein content. The plasma homocysteine concentration in rats fed wheat gluten was marketly less than casein,however plasma cysteine concentration in wheat gluten was higher than casein group.Conclusion The effects of wheat gluten on plasma homocysteine concentration are mainly depends on the low contents of methionine and high cysteine content,but the low contents of lyscine and threonine are not ignored. The mainly mechanism is that the increased cysteine concentration promot enzyme activities of homocystein metabolism,and increase the consumption of homocysteine.更多还原