【摘要】 目的探讨母鼠过量补充铁对仔鼠肝脏组织形态和功能的影响。方法将孕鼠按体重随机分为高、中、低剂量(120、60、30 mg/kg BW)补铁组和生理盐水对照组。选用右旋糖酐铁隔日对母鼠腹腔注射,共6周。测定血清铁含量,血清谷氨酸氨基转移酶(GPT)、天门冬氨酸氨基转移酶(GOT)活性;肝脏铁染色及病理组织学检查肝组织损伤情况,免疫组化法测定肝脏Bax和Bcl-2基因表达,氧化应激水平分析肝组织中丙二醛(MDA)含量、超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSHPx)活性。结果补铁6周后,高、中剂量组仔鼠血清铁水平均明显高于对照组(P<0.05)。低剂量组仔鼠肝组织有少量铁沉积,而中、高剂量组则有大量铁沉积,且随着肝脏铁蓄积的增多,肝细胞受损程度明显增加。中、高剂量组Bcl-2基因与Bax基因表达量的比值均明显低于对照组(P<0.05)。高剂量组肝ALT和AST活性均明显高于对照组(P<0.05)。中、高剂量组仔鼠肝组织MDA含量均明显高于对照组(P<0.05),GSH-Px活性均明显低于对照组(P<0.05);高剂量组SOD活性明显低于对照组(P<0.05)。结论孕期和哺乳期母鼠腹腔铁过量补充可导致仔鼠肝脏铁过量沉积,肝组织细胞和功能受到不同程度损伤,氧化应激水平明显提高。更多还原

【Abstract】 Objective To investigate the effects of iron overload on the liver tissue and function of maternal rats ’offspring. Methods Forty pregnant rats were randomly divided into four groups,high-dose group,middle-dose group,low-dose group( 120,60 and 30 mg / kg BW) and control group. Iron detran was administered by intraperitoneal injection every other day and the entire trial lasted for 6 weeks. After 6 weeks,the iron serum levels,GOT and GPT of rats’ offspring were determined. Histological changes of the liver injury were measured. The expressions of Bcl-2 and Bax in liver were measured by immunohistochemistry method. In addition,MDA,SOD and GSH-Px in liver were assessed by spectrophotometry. Results After 6 weeks,the level of serum iron in control group was significantly lower than in middle-dose and high-dose group( P < 0. 05),and the result of level of liver iron was similar with that. According to the HE staining,it showed that liver cell gradually was damaged with the increasing of accumulation ofhepatic iron. The expression of Bcl-2 / Bax was significantly higher in control group than in middle-dose and high-dose groups( P < 0. 05). The levels of serum GPT and GOT in highdose group were significantly higher in control group( P < 0. 05). The levels of Liver MDA in middle-dose and high-dose groups were both significantly higher than in control group( P < 0. 05). Moreover,the levels of liver GSH-Px and SOD in high-dose group were significantly lower than in control group( P < 0. 05). Conclusion Iron overload in maternal rats may result in excessive iron deposition and oxidative damage of liver tissue and cell function in maternal rats’ offspring.更多还原