【摘要】 目的探讨甲基硒酸(Me Se A)、硒代蛋氨酸(Se Met)和甲基硒代半胱氨酸(Me Se Cys)对宫颈癌He La细胞的增殖、迁移及粘附功能的影响。方法体外培养He La细胞,3μmol/L的Me Se A、Se Met和Me Se Cys处理He La细胞12~72 h,分别采用MTT法、划痕实验和体外基质粘附实验检测He La细胞的增殖、迁移及粘附功能。结果与对照细胞相比,Me Se A处理组He La细胞的增殖速度明显降低(P<0.01)。Me Se A处理组He La细胞在4 h和8 h的迁移抑制率分别为34%(P<0.05)和26%(P<0.05);Se Met处理组He La细胞在4 h和8 h的迁移抑制率分别为18%和13%;Me Se Cys处理组He La细胞在4 h和8 h的迁移抑制率分别为28%(P<0.05)和5%。Me Se A、Se Met和Me Se Cys对He La细胞粘附功能的抑制率分别为36%(P<0.01)、25%和49%(P<0.01)。结论 Me Se A可以更好地抑制He La细胞的增殖和迁移,而Me Se Cys对He La细胞的粘附功能的抑制作用更强。更多还原

【Abstract】 Objective To explore the effects of methylseleninic acid( MeSeA),selenomethionine( SeMet) and methylselenocysteine( MeSeCys) on proliferation,migration and adhesion of He La cells. Methods He La cells were cultured and treated with Me Se A,Se Met and Me Se Cys for 12- 72 h respectively. MTT assay,healing assay and in vitro cell Matrigel adhesion assay were used to detect the proliferation,migration and adhesion of He La cells. Results Compared to the control group,the proliferation of He La cells was remarkably inhibited by MeSeA( P < 0. 01). The migration of He La cells in MeSe A group was inhibited by 34%( P < 0. 05) and 26%( P < 0. 05) in 4 h and 8h,respectively. However,the migration of He La cells with inhibitions of 18% and 13%was in SeMet group in 4 h and 8 h. The inhibitions of He La cell migration in MeSeCys group was 28%( P < 0. 05) and 5% in 4 h and 8 h,respectively. In addition,theadhesive function of He La cells in the MeSeA group,the Se Met group as well as the Me Se Cys group were inhibited by 36%( P < 0. 01),25% and 49%( P < 0. 01).Conclusion The proliferation and migration of He La cell were effectively inhibited by MeSeA,while the adhesive function of He La cell was remarkably inhibited by MeSeCys.更多还原