【摘要】 目的探讨双酚A(BPA)对雄性小鼠睾丸和附睾中电压依赖性T型钙离子通道(Cav3)的影响及雌激素受体(ERs)的作用特点。方法将野生型(WT)小鼠、雌激素受体基因敲除(αERKO和βERKO)小鼠随机分为对照组和BPA组[100μg/(kg·d)],连续经口灌胃染毒30 d后,荧光定量PCR法检测睾丸和附睾中Cav3.1、Cav3.2和Cav3.3 mRNA表达的变化。结果 BPA可使小鼠睾丸中Cav3.1、Cav3.2和Cav3.3 mRNA表达水平升高,附睾中Cav3.1和Cav3.2 mRNA表达水平升高,但Cav3.3 mRNA表达水平降低。应用ERKO小鼠发现BPA通过ERα和ERβ影响附睾Cav3.1和Cav3.2 mRNA表达,对睾丸Cav3.1、Cav3.2和Cav3.3以及附睾Cav3.3 mRNA的调控则依赖于ERβ。结论 BPA可以影响雄性小鼠睾丸和附睾中电压依赖性T型钙通道3种亚型的基因表达,ERα和ERβ在此过程中发挥了重要作用。更多还原

【Abstract】 Objective To explore the possible effects of bisphenol A( BPA) on voltage-dependent T-type calcium channels in mouse testis and epididymis,the role of the two ERs was also investigated. Methods Adult male wild type( WT),ERs knock out mice( αERKO and βERKO) were randomly divided into control and BPA [100 μg /( kg·d) for 30 days via intragastric administration] treatment groups. Quantitative real time PCR was used to examine Cav3. 1, Cav3. 2 and Cav3. 3 genes of the testis and epididymis. Results In the testis,the mRNA expression of Cav3. 1,Cav3. 2 and Cav3. 3were increased in BPA-treated animals. In the epididymis,the mRNA expression of Cav3. 1 and Cav3. 2 were increased,however,Cav3. 3 were decreased in BPA-treated animals. BPA up-regulation of Cav3. 1 and Cav3. 2 mRNA expression of the epididymis were dependent on both ERα and ERβ,whereas BPA regulation of Cav 3. 1- Cav3. 3 ofthe testis and Cav3. 3 of the epididymis appeared to be dependent on ERβ only.Conclusion It suggested that the ERs signalling was necessary for the regulation of the Cav3 channels mRNA expression by BPA,which could affect the male reproductive function.更多还原