[Objective] Dam H is a bifunctional hydrolase that hydrolyzes the amide and ester bonds. Previous studies demonstrated that mutagenesis of non-catalytic residues shows a negative impact on the soluble expression and specific activity of Dam H. Therefore,we studied the catalytic triad of Dam H and the effect of non-catalytic mutagenesis on the soluble expression and specific activity of Dam H. [Methods] We performed site-directed mutation experiment of the 3possible catalytic sites: S149,E244 and H274 and th...
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