【目的】筛选获得印度洋海水解有机磷细菌,研究其解有机磷作用机理,初步了解该地区可培养有机磷细菌的系统发育多样性。【方法】采用卵磷脂培养基从分离自印度洋的细菌中筛选解有机磷细菌,根据16S r RNA基因序列确定获得的有机磷细菌的分类地位;同时挑选解磷效果显著的3株细菌利用液体发酵进行产酶和解磷特性分析。【结果】自916株细菌中得到99株具有解有机磷活性的细菌,分属于16个属。在以卵磷脂为其有机磷来源时,菌株India-BSP-1(Cobetia sp.)、India-BSP-21(Pelagibaca sp.)、India-BSP-23(Pelagibacterium sp.)的培养液中磷酸盐(DIP)浓度曲线为N型,并且碱性磷酸酶的检测活性滞后于DIP的生成。【结论】印度洋海水中解有机磷细菌种类多样性丰富,疑似有新种;解有机磷细菌的解磷特性受DIP和碱性磷酸酶相互作用的影响。
【英文摘要】
[Objective] The aim of this study is to screen culturable phosphate-solubilizing bacteria from water samples of the Indian Ocean, and tackle their mechanism of regenerating phosphate from organics and their phylogenetic diversity in the region. [Methods] Phosphate-solubilizing bacteria were selected by inoculating bacteria isolated from the Indian Ocean waters onto solid lecithin medium, and the phosphate-solubilizing bacteria were identified according to their 16 S r RNA genes. Three strains of high phosph...
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