[Objective] We established the mutation library of pathogenic Vibrio parahaemolyticus(Vp), analyzed the phenotype characteristic of hemolytic ability changed mutations, to provide the research basis for deeper exploration and understanding of the regulation system and mechanism of tdh. [Methods] In this study, the Mini-Tn5-Km2 was inserted randomly into the genome of pathogenic Vp(ATCC 33846) by biparental mating. The conjugants(KmRAmpS) were investigated using thiosulfate citrate bile salts sucrose agar me...