【摘要】 以cis-[Ru(dpq)2Cl2]·2H2O(dpq=二吡啶[3,2-d∶2′,3′-f]二氮萘)为原料与5,5′-二(1-(三乙胺)甲基)-2,2′-联吡啶阳离子(L)合成钌(Ⅱ)配合物[Ru(dpq)2L](PF6)4,并研究了该配合物与G-四链体DNA的作用:FRET实验表明,配合物对人端粒DNA h-telo具有选择性,其作用能力要强于同癌基因启动子区域的四链DNA,如c-myc和bcl2;CD光谱表明,在Na+和K+都不存在的情况下,配合物能诱导h-telo形成平行结构;此外,紫外和发射光谱都显示,配合物在K+溶液中与h-telo的作用力要大于在Na+溶液中的。更多还原

【Abstract】 Based on cis-[Ru(dpq)2Cl2]·2H2O(dpq=dipyrido[3,2-d∶2′,3′-f ]quinoxaline) and 5,5′-di(1-(triethylammonio)methyl)-2,2′-dipyridyl cation ligand(L), the complex [Ru(dpq)2L](PF6)4was synthesized and structurally characterized. The interactions of the complex with different G-quadruplexes were investigated. FRET melting assay proved that the complex bonds more strongly to h-telo than to promoters, such as c-myc and bcl2. CD studies show that Ru (Ⅱ) complex can induce the formation of parallel G-quadruplex of h-telo in the absence of Na+or K+. Results of absorption and emission titration indicated that the complex has a higher DNA affinity with h-telo in K+buffer than in Na+buffer. CCDC: 945364.更多还原