【摘要】 目的研究趋化因子CXCL12对胃癌BGC-823细胞体外侵袭、转移能力的影响,进一步探讨CXCL12/CXCR4轴在胃癌侵袭、转移过程中的机制。方法采用Transwell法、real-time PCR法及免疫荧光法分别检测经不同浓度CXCL12处理后的胃癌BGC-823细胞侵袭能力的变化、MMP-2 mRNA表达量的变化、MMP-2蛋白表达的变化。结果与空白对照组比较,CXCL12不同浓度组穿膜细胞数和MMP-2 mRNA表达量均显著增多(P<0.001)。上述指标在CXCL12不同浓度组间比较,除200 ng/ml组与100 ng/ml组间相比,差异无统计学意义(P>0.05)外,其余各组间比较,差异均有统计学意义(P<0.001);免疫荧光结果显示,随着CXCL12浓度的升高,胃癌BGC-823细胞MMP-2蛋白表达增强。结论 CXCL12能增强胃癌BGC-823细胞的体外侵袭能力,并具有一定的量效关系,其促进胃癌侵袭、转移的机制可能与增强MMP-2的表达有相关性。更多还原

【Abstract】 Objective To study the effects of chemokine CXCL12 in vitro invasion and metastasis of gastric cancer BGC-823 cells,and further explore the CXCL12 / CXCR4 axis possible mechanism of invasion and metastasis in gastric cancer. Methods Transwell method,real-time PCR assay and immunofluorescence were used to detect the changes of gastric cancer BGC-823 cells invasion ability,expression level of MMP-2 mRNA,expression of MMP-2 protein after treatment with different concentrations of CXCL12. Results Compared with the blank control group,the number of invasive cells and the expression of MMP-2 mRNA were significantly increased in different concentrations of CXCL12 groups(P < 0. 001). These indicators had significant statistical difference among different concentrations of CXCL12 groups(P< 0. 001),except no significant difference between 100 ng / ml group and 200 ng / ml group( P > 0. 05). Immunofluorescence showed that with increasing concentrations of CXCL12,expression of MMP-2 protein in gastric cancer BGC-823 cells was enhanced. Conclusion CXCL12 can increase the invasion of gastric cancer BGC-823 cells in vitro,and has a certain dose-effect relationship,the mechanism of invasion and metastasis may be related to its enhancement of MMP-2expression.更多还原