【摘要】 以总固体1∶1的玉米秸秆和猪粪为混合原料,采用10 L厌氧反应器进行批式全混中温沼气发酵,并利用454焦磷酸测序法测定混合发酵过程中细菌群落变化。结果表明:预处理玉米秸秆和猪粪混合沼气发酵启动迅速,在第4天达到产气高峰,容积产气率为1.17 L/(L·d),46 d原料沼气产率为356.55 m L/g VS。该混合发酵系统中共有23~27门细菌,其中优势菌群为拟杆菌门(Bacteroidetes)、厚壁菌门(Firmicutes)和变形菌门(Proteobacteria)。发酵期内的Shannon指数在4.88~5.53之间,说明发酵期内细菌多样性比较稳定。更多还原

【Abstract】 Using pretreated corn stalks and pig manure(total solid ratio 1∶1)as mixed materials,10 L anaerobic reactorwas used to carry out the mesophilic batch co-fermentation for biogas production;The 454 pyrosequencing was used tomeasure change of bacteria community in mixed fermentation process. The results show that the start- up of the cofermentation of pretreated corn stalks and pig manure is very quickly. The daily biogas yield reached the maximum of1.17 L/(L·d) in 4th day. The biogas yield was 356.55 m L/g VS in 46 days. There were 23-27 different bacteria Phylum inthe co-fermentation system and the dominant phyla were Bacteroidetes,Firmicutes and Proteobacteria among them. TheShannon index during the co- fermentation process was between 4.88-5.53. It indicated the diversity of bacteria wasrelatively stable in co-fermentation period.更多还原