【摘要】 目的分析葡萄籽乙醇提取液对医院临床分离菌株的体外抑菌作用和毒性。方法以临床标本分离菌株为对象,采用抑菌环试验和营养肉汤稀释法对其抑菌效果进行了试验观察;采用动物试验对其进行了毒性试验观察。结果含葡萄籽生药125 g/L的乙醇提取液对临床分离菌株和标准菌株的抑菌环直径均大于7 mm。葡萄籽乙醇提取物对白色念球菌的最低抑菌浓度(MIC)为25.0 g/L。小鼠急性经口LD50值大于5000 mg/kg,葡萄籽提取原液对家兔皮肤刺激试验指数为0.13,属于轻度刺激性。结论葡萄籽乙醇提取液具有良好的抑菌作用,属无毒、低刺激性物质。更多还原

【Abstract】 Objective: To analyze the bacteriostatic efficacy and toxicity of grape seeds ethanol extract in hospital clinically isolated strain. Methods: Clinically isolated strain specimens were used to observe the bacteriostatic efficacy of grape seed ethanol extract via bacterial inhibition zone test and nutrient broth method; and animal test was employed to observe experimentally its toxicity. Results: The diameters of bacterial inhibition zones of clinically isolated microorganism strain and standard microorganism strain exposed to the ethanol containing grape seed crude drug 125 mg / L were larger than 7mm. The minimum inhibition concentration( MIC) of grape seed ethanol extract for C albicans was 25. 0 mg / ml. The mouse acute oral LD50 was above 5000 mg / kg,the rabbit skin irritation test index of grape seed stock solution was 0. 13,which belonged to mild irritation. Conclusion: Grape seed extract has good bacteriostatic efficacy and it belongs to practically non-toxic class and was slightly irritative to skin.更多还原