【摘要】 目的探讨壬基酚对卵巢癌细胞HO8910增殖的作用。方法 HO8910细胞在有酚红RPMI1640(含10%新生牛血清)培养液中常规培养,实验前1天改为无酚红RPMI1640(含5%CDT-FBS)中继续培养,1×10-5mol/L,1×10-6mol/L,1×10-7mol/L的NP和1×10-11mol/L的E2处理细胞24 h后,MTT法和流式细胞术检测对HO8910细胞的增殖作用。结果 1×10-5mol/L,1×10-6mol/LNP组和E2组细胞增殖率和细胞增殖指数均有明显增加。结论壬基酚对HO8910细胞发挥雌激素样作用,有促进卵巢癌细胞增殖的作用。更多还原

【Abstract】 Objective: To investigate the effect of nonylphenol on ovarian cancer HO8910 cells proliferation. Method:HO8910 cells was maintained in RPMI1640 medium with 10% neonatal bowine serum. 1d before the beginning of the experiment,the cells were be seeded in phenol red- free 1640 medium containing 5% charcoal dextran- treated FBS,after 1 ×10- 5mol / L,1 × 10- 6mol / L and 1 × 10- 7mol / L of NP and 1 × 10- 7mol / L E2 treatment for 24 h,the proliferation of HP8910 was analyzed by MTT and flow sytometer. Results: Cell proliferation rate and Cell proliferation index increased significantly in 1 × 10- 5mol / L,1 × 10- 6mol / LNP groups and E2 group. Conclusion: NP produce similar effects on HO8910 with E2,maybe promote the proliferation ovarian cancer cell.更多还原