【摘要】 探讨秀丽隐杆线虫天然粗提物的提取及对哮喘动物治疗效果。将线虫经离心、洗涤、超声乳化、去除内毒素等方法获得线虫粗提物(CEC),以鸡卵清蛋白(OVA)致敏BALB/c雌性小鼠建立哮喘动物模型,用CEC与OVA混合物(CE)对哮喘动物治疗,以缓冲液(PBS)和OVA为对照,用ELISA法检测血清Ig E、Ig G1、Ig G2a抗体,分类计数支气管肺泡灌洗液(BALF)细胞,切取肺组织观察病理。研究显示,CE组与OVA组比较,血清Ig E、Ig G1、Ig G2a差异有统计学意义(P<0.05),BALF中细胞总数、嗜酸性粒细胞、巨噬细胞等差异有统计学意义(P<0.05)。线虫天然粗提物对哮喘动物有一定治疗效果。更多还原

【Abstract】 To investigate the extraction of Caenorhabditis elegans and its effect on curing animals with allergic asthma.The crude extract of C. elegans(CEC) was obtained through centrifugation,washing,phacoemulsification and endotoxin removal. Sensitizing BALB/c mice with ovalbumin(OVA) was used to develop the animal model of asthma. Animals with asthma were treated with a mixture of CEC and OVA(CE). Buffer solution(PBS) group and OVA group were set as control groups. The serum levels of Ig E,Ig G1 and Ig G2 a were measured with commercial ELISA kits,and the quantity of cells in the bronchoalveolar lavage fluid(BALF) were counted. Lung tissues were removed to observe its histopathology. The results showed that the differences in the serum levels of Ig E,Ig G1 and Ig G2 a of CE group and OVA group were statistically significant(P < 0. 05). The differences in the quantity of total cells,eosinophils and macrophages in BALF were statistically significant(P < 0. 05). It showed that CEC have therapeutic effect on animals with allergic asthma.更多还原