【摘要】 目的探讨野黄芩苷诱导人舌鳞癌Tca8113细胞凋亡的可能机制。方法将体外培养的人舌鳞癌Tca8113细胞分成对照组和不同浓度野黄芩苷组(浓度分别为80、120、160 mg/L),每组设3个复孔。采用Tunel检测细胞凋亡情况;采用免疫细胞化学法和Western blot法检测caspase-8蛋白表达情况。结果 (1)80、120、160mg/L野黄芩苷组Tca8113细胞凋亡率高于对照组[(17.63±0.25)%、(36.23±0.36)%、(51.84±0.58)%vs(4.45±0.27)%,P<0.05]。(2)与对照组相比,80、120、160 mg/L野黄芩苷组caspase-8蛋白的表达增加(0.283±0.040 vs0.474±0.031、0.592±0.077、0.781±0.020,P<0.05)。结论野黄芩苷能明显诱导舌鳞癌细胞Tca8113的凋亡,可能与上调caspase-8蛋白表达有关。更多还原

【Abstract】 Objective To investigate the possible mechanism of scutellarin on the apoptosis of human tongue squa-mous carcinoma Tca8113 cells. Methods Human tongue squamous carcinoma Tca8113 cells were divided into controlgroup and scutellarin groups(80, 120 and 160 mg/L). Tunel method was used to detect the apoptosis. Immunohistochemistryand Western blot assay was used to detect the caspase-8 protein expression of cells. Results(1)The apoptotic rates ofTca8113 cells were significantly high in scutellarin groups(80, 120 and 160 mg/L) than those in control group [(17.63±0.25)%,(36.23±0.36)%,(51.84±0.58)% vs(4.45±0.27)%, P < 0.05].(2)Compared with control group, the expressions of cas-pase-8 protein were significantly increased in different concentrations(80, 120 and 160 mg/L) of scutellarin groups(0.283±0.040 vs 0.474±0.031, 0.592±0.077, 0.781±0.020,P < 0.05). Conclusion Scutellarin could obviously induce the apoptosisof human tongue squamous carcinoma Tca8113 cells, which may be related to the caspase-8 protein expression.更多还原