【摘要】 目的:探讨不同时间持续缺氧孵育后脂肪细胞脂联素和瘦素表达的变化。方法:将分化成熟的3T3-L1脂肪细胞随机分为6组,即常氧(21%O2)组和缺氧(1%O2)4、12、24、48、72 h组,实时定量PCR(q RT-PCR)法测定各时间点脂肪细胞中缺氧诱导因子1α(HIF-1α)、葡萄糖转运子1(GLUT-1)、脂联素和瘦素基因表达。结果:与常氧组相比,缺氧12、24、48 h组HIF-1α、GLUT-1 m RNA表达水平升高,差异有统计学意义(P<0.05)。缺氧24 h时HIF-1α、GLUT-1 m RNA达到最高。各缺氧组脂联素m RNA在12、24、48、72 h缺氧组的表达下降,较常氧组有统计学差异(P<0.05)。各缺氧组瘦素m RNA的表达高于常氧组,并在24 h时达到最高,差异有统计学意义(P<0.05)。结论:缺氧使脂肪细胞HIF-1α、GLUT-1、瘦素m RNA表达升高,脂联素m RNA表达下降,各反应在缺氧24 h时达到最大水平。更多还原

【Abstract】 O bjective:To explore the effect of different incubation time of hypoxia on the expression of adipokines in the 3T3-L1 adipocytes. Methods: The differentiated 3T3-L1 adipocytes were randomly divided into 6 groups, normoxia(21%O2)and hypoxia(1%O2)conditions for 4, 12, 24, 48, 72 h respectively. The m RNA expressions of hypoxia inducible factor-1α(HIF-1α), glucose transporter 1(GLUT-1), adiponectin and leptin were detected by the q RT-PCR. Results: In 3T3-L1 adipocytes, the m RNA expressions of HIF-1α and GLUT-1 in hypoxia conditions for 12 h, 24 h and 48 h were higher than normoxia-treated cells(P<0.05); furthermore, they were highest in the group of hypoxia-treated cells for 24 h. Compared with the normoxia group, the adiponectin m RNA expressions of the hypoxia-treated3T3-L1 adipocytes decreased in all groups(P<0.05) except for the 4 h group(P<0.05). Ttranscription level of leptin in the hypoxia-treated cells increased(P<0.05), and it reached the highest level in the 24 h hypoxia group. Conclusion: The m RNA expressions of HIF-1α, GLUT-1 and leptin of 3T3-L1 adipocytes increase in the condition of hypoxia incubation, while the level of adiponectin m RNA decreases under hypoxia treatment. All changes above reach the peaks within the 24 h of hypoxia-treated 3T3-L1 adipocytes.更多还原