【摘要】 建立了简便、快速、灵敏的测定苹果和土壤中虱螨脲残留的超高效液相色谱分析方法。样品采用乙腈提取,提取液经盐析后无需净化,将上清液浓缩复溶,过膜后直接上机测试。试样采用BEH C18(50.0 mm×2.1 mm,1.7μm)色谱柱分离,甲醇-水(60∶40)为流动相等度洗脱后,紫外检测器检测,检测波长为255 nm。结果表明,虱螨脲在0.025~2.5 mg/L浓度范围内线性关系良好,相关系数为0.999 7。在0.05~0.5 mg/kg加标浓度范围内的平均回收率为84.5%~98.7%,相对标准偏差(RSD)为0.7%~6.1%,方法的检出限为0.01 mg/kg。更多还原

【Abstract】 A quantitative method using ultra performance liquid chromatography( UPLC) with UV detector was developed for the determination of lufenuron residue in apple and soil. The sample was extracted with acetonitrile and the extractants were separated from water caused by the addition of salts.The supernatant was concentrated to dryness without any further clean-up step,and the residue was redissolved with methanol and then diluted with water prior to HPLC analysis. A BEH C18column( 50. 0 mm × 2. 1 mm,1. 7 μm) was used to separate the sample by using a mixture of methanol-water( 60 ∶ 40) as mobile phase at a flow rate of 0. 4 m L / min. The ultraviolet detection wavelength was set at 255 nm. The method had a good linear relationship in the range of 0. 025- 2. 5 mg / L with a correlation coefficient( r) of 0. 999 7. The average recoveries of lufenuron at a spiked range of 0. 05- 0. 5 mg / kg ranged from 84. 5% to 98. 7% with relative standard deviations( RSDs) of 0. 7%-6. 1%. The limit of detection( LOD) was 0. 01 mg / kg.更多还原