【摘要】 采用压力驱动的亲和毛细管电泳技术(P-ACE)分别研究了生理酸度条件下(p H 7.4)3种黄酮类化合物龙血素A、龙血素B和剑叶龙血素C与人血清白蛋白(HSA)之间的相互作用。利用蛋白淌度变化和药物浓度的关系,计算得出上述三者与HSA的结合常数Ka分别为0.414×105,0.252×105,1.816×105L/mol。结果表明,P-ACE可作为研究药物与蛋白相互作用的简便可行方法。更多还原

【Abstract】 The interactions of loureirin A( LA),loureirin B( LB) and cochinchinenin C( CC) with human serum albumin( HSA) under simulated physiological conditions( p H 7. 4) were studied by pressure-mediated affinity capillary electrophoresis method( P- ACE). The binding constants were0. 414 × 105,0. 252 × 105,1. 816 × 105 L/mol for LA, LB and CC, respectively. The results showed that P- ACE was a useful method to study the interaction between drug and protein.更多还原