【摘要】 建立了同时测定川贝枇杷糖浆中6种有效成分(桔梗皂苷D、贝母辛、齐墩果酸、西贝素、贝母甲素和贝母乙素)含量的微流蒸发光散射检测器(μELSD)与加压毛细管电色谱(p CEC)联用检测方法。搭建p CEC-μELSD联用平台,采用C18毛细管色谱柱(EP-150-15-3-C18),流动相为乙腈(A)-10 mmol·L-1甲酸-三乙胺水溶液(p H 11.0)(B),梯度洗脱,柱流速为1.02μL·min-1,施加电压为+10 k V,雾化载气流速为0.4 L·min-1,蒸发温度为40℃。川贝枇杷糖浆中6种有效成分可在10 min内得到分离检测,6种有效成分的线性范围可达4个数量级,检出限均在pg水平,加标回收率为97.9%~103.0%。所建立的p CEC-μELSD联用方法精密度、重复性和稳定性良好,且简便、快速、可靠,可用于川贝枇杷糖浆中6种有效成分的含量测定。更多还原

【Abstract】 Microfluidic evaporative light scattering detector( μELSD) coupled to pressurized capillary electrochromatography( p CEC) was applied in the separation and detection of six active components,e. g. platycodin D,peimisine,oleanolic acid,imperialine,peimine and peiminine in Chuanbei Pipa Syrups. The simultaneous analysis of six active components by p CEC-μELSD was performed on a C18 capillary column( EP-150-15-3-C18) with mobile phase A: acetonitrile and mobile phase B: 10 mmol·L-1formic acid-triethylamine aqueous solution( p H 11. 0) by gradient elution. The optimal conditions were as follows: column flow rate: 1. 02 μL·min-1,applied voltage: + 10 k V,nebulizing gas flow rate: 0. 4 L·min-1and evaporative temperature: 40 ℃. Six active components in Chuanbei Pipa Syrups were successfully separated in 10 min with the excellent linearity of four orders of magnitude. The limit of detection( LOD) could reach up to the level of pg,and the recoveries were between 97. 9% and 103. 0%. The precision,repeatability and stability indicated a good reproducibility for the determination. The results also demonstrated the feasibility and practicality of p CEC-μELSD platform. The method could be used for the determination of the active ingredients in Chuanbei Pipa Syrups.更多还原