【摘要】 目的:筛选溃疡性结肠炎相关性结肠癌与正常组织表达的差异蛋白。方法:应用蛋白质组学技术,对小鼠结肠癌组织和正常对照组织进行胶内差异双向电泳、(2-D),选择差异表达超过2倍的蛋白点进行MALDI-TOF-MS质谱分析。结果:成功建立结肠癌和对照组织的双向凝胶电泳图谱;对其中差异表达超过2.0倍蛋白质点7个进行了质谱分析和数据库检索鉴定。结论:溃疡性结肠炎相关性结肠癌存在差异表达的蛋白,有可能成为该病潜在的、有应用价值的分子标志。更多还原

【Abstract】 Objective To screen differentially expressed proteins in ulcerative colitis related colorectal cancer(UC-CRC).Methods Two-dimensional electrophoresis was applied to separate the proteins in mouse colorectal cancer and normal tissues specimens.The proteins expressed differently of a 2-fold change were cut and identified by MALDI-TOF-MS. Results Twodimensional protein maps of colorectal cancer and normal tissues were gained successfully. There were 7 protein spots of a 2-fold change which were identified by using mass spectrometry. Conclusion there are differentially expressed proteins in UC-CRC, which may become potential and valuable molecμLar markers for UC-CRC.更多还原