【摘要】 目的探讨改良三甲散对AD大鼠细胞模型NF-κB和JNK信号传导通路的调节作用。方法选用健康大耳白兔分高、中、低剂量连续6天给药,抽取含药脑脊液备用。选用出生24 h内乳大鼠取海马神经细胞做原代培养,采用终浓度为5μmol/L的Aβ25-35诱导造模,用改良三甲散含药脑脊液处理AD细胞模型,采用ELISA测定细胞上清液中NF-κB和JNK的表达情况。结果改良三甲散能显著降低细胞模型上清液中NF-κB和JNK的含量(P<0.05)。结论改良三甲散治疗老年性痴呆病的机制可能与抑制NF-κB和JNK信号转导通路的激活有关。更多还原

【Abstract】 Objective To discuss the regulation of Gai Liang San Jia San in NF- κB and JNK signaling pathway of AD cell model on rats. Methods Choosing healthy rabbits,divided into high,medium and low dose group,6 days administered continuously,then extract the drug- containing cerebrospinal fluid backup. Choosing neonatal rat within 24 hours of birth,taking hippocampal neurons for primary culture. Modeling using Aβ25- 35 which final concentration is 5μmol / L. Treating AD cell model with drug-containing cerebrospinal fluid. Determining the expression of NF- κB and JNK in cell supernatant. Results Gai Liang San Jia San can significantly reduce the content of NF- κB and JNK in cell model supernatant( P < 0. 05). Conclusion Mechanism of Gai Liang San Jia San in treatment of Alzheimer’s disease may concerning inhibition of NF- κB and JNK signal transduction pathway activation.更多还原