【摘要】 目的:观察右美托咪定(Dex)对离体培养的人中性粒细胞(PMN)凋亡率和呼吸爆发功能的影响。方法:取健康人外周静脉血,血浆-Percoll不连续密度梯度离心法分离出PMN,分为对照组,1 ng/m L Dex组,10ng/m L Dex组和100 ng/m L Dex组,离体培养24 h,取培养2 h和24 h的PMN悬液,检测caspase-3活性、PMN凋亡率和呼吸爆发功能。结果:与对照组比较3种不同浓度的Dex与PMN孵育2 h,PMN的caspase-3活性、PMN凋亡率和呼吸爆发功能没有统计学差异;孵育24 h,与对照组比较,1 ng/m L Dex组PMN caspase-3活性、PMN凋亡率和呼吸爆发功能没有差异,而10 ng/m L Dex组和100 ng/m L Dex组PMN caspase-3活性增强、凋亡率增加和呼吸爆发功能下降;与10 ng/m L De组比较,100 ng/m L Dex组PMN caspase-3活性增强、凋亡率增加和呼吸爆发功能下降。结论:临床常用剂量的Dex(血药浓度<1 ng/m L)不影响PMN的凋亡率和呼吸爆发功能,不影响机体的抗感染能力;而大剂量的Dex与PMN孵育一定时间(>24 h)可促进PMN凋亡,降低其呼吸爆发功能。更多还原

【Abstract】 Objective To investigate the effect of dexmedetomidine(Dex) on the apoptosis and respiratory burst of human polymorphonuclear neutrophils(PMN) in vitro. Methods The peripheral venous blood was collected from healthy volunteers. Isolation of PMN was performed by using the discontinuous plasma-Percoll gradient technique. PMN was random Ly divided into four groups: control group and three different concentrations of Dex(1 ng / m L,10 ng / m L,100 ng / m L) groups. PMN was cultured in enriched RPMI-1640 media at 2 × 106/ m L for 24 h. The caspase-3 activity, apoptosis rate and respiratory burst of PMN were detected at 2 and 24 h.Results Compared with the control group, the PMN which was treated with three different concentrations of Dex showed no significant difference in caspase-3 activity, apoptosis rate and respiratory burst of PMN after 2 h of incubation. Compared with the control group, 1 ng / m L concentration of Dex did not affect the caspase-3activity, apoptosis and respiratory burst of PMN cultured for 24 h. However, 10 ng / m L and 100 ng / m L concentrations of Dex promoted the caspase-3 activity, increased apoptosis rate and reduced the respiratory burst of PMN after 24 h of incubation. Compared with 10 ng / m L Dex group, 100 ng / m L concentrations of Dex promoted the caspase-3 activity, increased apoptosis rate and reduced the respiratory burst of PMN after 24 h of incutation.Conclusion Clinically relevant concentration of Dex does not affect apoptosis and respiratory burst of PMN,while high concentration of dexmedetomidine can induce apoptosis of PMN.更多还原