【摘要】 为了明确不同番茄砧木的抗南方根结线虫特性,及抗性与生理指标、土壤生物学活性间的关系,采用温室盆栽人工接种法,鉴定了12个番茄砧木对南方根结线虫(Meloidogyn incognita)的抗性程度,测定番茄叶片防御酶活性和根系活力、相对电导率、叶绿素、脯氨酸、丙二醛等生理指标,采用连续稀释法测定土壤中微生物的种类、数量,并测定土壤酶活性,同时分析上述指标与病情指数的相关性。试验结果表明:番茄砧木Gardna,Baliya,Greengard,Anka-T,Volante和9748表现为高抗;M158和RS2表现为中抗;Kagemusha和RS1表现为抗病;Vespa表现为感病;L-402表现为高感。相关分析结果表明,番茄砧木抗南方根结线虫能力与叶片PPO和POD活性在0.01水平上显著正相关,相关系数分别为0.708和0.681,与叶片PAL活性在0.05水平上显著正相关,而与叶片相对电导率和丙二醛含量在0.05水平上显著负相关。在本试验土壤环境条件下,不同番茄砧木根际土壤细菌、放线菌数量,土壤脲酶、过氧化氢酶、多酚氧化酶、过氧化物酶活性随抗南方根结线虫特性增强而呈增加趋势。上述指标的生理生化过程可作为番茄抗线虫机理研究的重点方向。更多还原

【Abstract】 Twelve tomato rootstocks were inoculated artificially with M. incognita to identify resistance index in greenhouse. The relative electric conductivity, root vigor and total chlorophyll, soluble protein, proline, malonaldehyde(MDA), and the activities of phenylalanine ammonia lyase(PAL), polyphenol oxidase(PPO), peroxidase(POD) in different tomato leaves were measured. The microorganism quantity in rhizosphere soil was investigated by the serial dilution method, and the enzymes activities in rhizosphere soil were determined by spectrophotometry or colorimetric titrations. The results showed that tested rootstocks ’Gardna’, ’Baliya’, ’Greengard’, ’Anka-T’,’Volante’ and ’9748’ showed high resistance, ’M158’ and ’RS2’ showed moderate resistance, ’Kagemusha’ and ’RS1’ showed resistance,’Vespa’ showed susceptible, and ’L-402’ showed high susceptible to M. incognita. Correlation analysis results showed that tomato rootstock resistance to M. incognita was highly correlated with the activities of PPO and POD in leaves with correlation coefficients of0.708 and 0.681. Meanwhile, the resistance was significantly(at 0.05 level) correlated with the activities of PAL in leaves. But, the relative electric conductivity and the content of MDA in blade were significant(at 0.05 level) negatively correlated with resistance. In this soil environment, the quantity of bacteria and actinomyces, together with the activities of urease, catalase, polyphenol oxidase and peroxidase, increased with higher resistance to M. incognita. The physiological and biochemical process of above factors could be an important direction of researching tomato resistance to M. incognita.更多还原