【摘要】 目的研究人子宫内膜癌组织中是否存在血管生成拟态(VM),及其与血管内皮生长因子(VEGF)、环氧化酶-2(COX-2)表达的关系。方法收集子宫内膜癌标本60例及临床病理资料,CD34-PAS双重染色证实VM存在,分析VM与患者临床病理指标之间的关系。进一步对60例子宫内膜癌标本进行免疫组化技术检测VEGF、COX-2的表达水平并比较其在有VM组与无VM组之间的差异来探讨其在VM形成过程中的作用。结果 13例标本中存在VM,阳性率21.7%。具有VM的子宫内膜癌恶性程度高,易发生转移。VEGF、COX-2表达强度与子宫内膜癌的病理分级呈正相关(P<0.05)。存在VM的子宫内膜癌中VEGF、COX-2表达水平更高(P<0.05)。结论子宫内膜癌组织标本中存在VM;VM与子宫内膜癌的侵袭、转移密切相关;VEGF、COX-2在子宫内膜癌VM形成过程中发挥着重要调节作用。更多还原

【Abstract】 Objective This experiment is to study whether vasculogenic mimicry is the special model of tumor blood vessels exists in endometrial carcinoma and the relationship of endometrial carcinoma vasculogenic mimicry with microvessel density VEGF,COX- 2 expression.Methods Specimens of 87 cases were collected along with their clinicopathologic data. VM channels were detected using CD34 / PAS double staining and were then used to study the correlation between VM and clinicopathologic factors. Immunohistochemical staining were performed to these endometrial carcinoma patients,we studied the expression of VEGF,COX- 2. Staining index( SI) was assessed to investigate the different expression between VM- positive group and VM- negative group to study the effect in the formation of VM. Results 13 cases have VM of the all60 patients( positive rate 21. 7%). The endometrial carcinoma cases belonging to VM group with higher invasive,had a higher rate of hematoge-nous metastases( P < 0. 05). As the differentiated degree degrades,the expression of VEGF,COX- 2 proteins was positive correlation( P < 0.05). The expression of VEGF,COX- 2 proteins in the VM group was significantly higher than that in the non- VM group( P < 0. 05). Conclusion VM exist in endometrial carcinoma. VM is related to invasion and metastasis of endometrial carcinoma. VEGF,COX- 2 may play an important regulating role in the formation of VM in vasculogenic mimicry.更多还原