【摘要】 目的通过过表达miRNA-19b,研究miRNA-19b对P19细胞分化的影响。方法脂质体2000转染miRNA-19b过表达质粒与空载质粒进入P19细胞,通过杀稻瘟菌素筛选出稳定株;实时定量聚合酶链反应检测miRNA-19b表达水平以及分化基因(c Tn T、ANP、GATA4)的表达水平。结果 miRNA-19b过表达载体与细胞系成功建立(P<0.05);在P19细胞分化过程中,miRNA-19b过表达组心肌标志物均显著高于对照组(P均<0.05)。miRNA-19b显著促进P19细胞向心肌细胞分化。结论 miRNA-19b可以显著促进P19细胞向心肌细胞的分化,为以后其用于心脏发育的进一步研究奠定基础。更多还原

【Abstract】 Objective The aim of this study is to explore the effects of miRNA- 19 b on P19 cell differentiation. Methods We transfected miRNA- 19 b overexpression plasmid and empty plasmid into P19 cells by lipo2000. The stable cell lines was selected by blasticidin. The expression of miRNA- 19 b and differentiation genes were detected by real- time quantitative PCR. Results The miRNA- 19 b overexpression was successfully established( P < 0. 05). Compared with negative control group,the overexpression of miRNA- 19 b promoted the differentiation of P19 cells significantly( P < 0. 05). Conclusion The miRNA- 19 b significantly promotes the differentiation of P19 cells,which contribute to the further study of the heart development.更多还原